Product Info Summary
SKU: | A10444 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Uplc1/Asap3 Antibody
SKU/Catalog Number
A10444
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Uplc1/Asap3 Antibody (Catalog # A10444). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store UPLC1 / ASAP3 Antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Uplc1/Asap3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10444)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant human UPLC1/ASAP3 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10444 is reactive to ASAP3 in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
99155 MW
Background of ACAP4
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Anti-UPLC1 (up-regulated in liver cancer 1) / ASAP3 Antibody, also named DDEFL1 (development and differentiation-enhancing factor-like 1) or ASAP3, is a member of the AZAP family of proteins. These proteins catalyze the hydrolysis of GTP bound to ADP-ribosylation factor (Arf) proteins, thereby causing Arf inactivation. For this reason, the ASAPs are generally called ArfGAPs. The activity of ArfGAPs is dependent on the presence of phosphoinositides and is implicated in cellular processes such as membrane trafficking and remodeling of the actin cytoskeleton. ASAP3 has been found to be up-regulated in 80% of the hepatocellular carcinomas examined. Initial biochemical characterization reveals that ASAP3 shows class-specific GAP activity on Arf proteins, preferring Arf5 over Arf1 and Arf6. ASAP3 antibody has beed developed through the NCI antibody collaboration program and is ideal for Cancer and Signal Transduction research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10444 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:1,000 - 1:10,000
WB: 1:200 - 1:2,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of UPLC1/ASAP3 expression in NIH/3T3 cells over-expressing the protein using rabbit anti-UPLC1/ASAP3 protein A purified polyclonal antibody (Catalog # A10444) at 1:10,000. Personal Communication, Vi Luan HA, CCR-NCI, Bethesda, MD.
Protein Target Info & Infographic
Gene/Protein Information For ASAP3 (Source: Uniprot.org, NCBI)
Gene Name
ASAP3
Full Name
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3
Weight
99155 MW
Alternative Names
UPLC1, ASAP3, UPLC1/ASAP3, Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3, FLJ20199, Gm140, Development and differentiation-enhancing factor-like 1, Protein up-regulated in liver cancer 1, DDEFL1 ASAP3 ACAP4, CENTB6, DDEFL1, UPLC1 ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3|ARF6 GTPase-activating protein|ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 1|centaurin, beta 6|development and differentiation enhancing factor-like 1|protein up-regulated in liver cancer 1|up-regulated in liver cancer 1 (UPLC1)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ASAP3, check out the ASAP3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ASAP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Uplc1/Asap3 Antibody (A10444)
Hello CJ!
A10444 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
MicroRNA‐143‐3p inhibits colorectal cancer metastases by targeting ITGA6 and ASAP3
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Customer Q&As
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1 Customer Q&As for Anti-Uplc1/Asap3 Antibody
Question
We are currently using anti-Uplc1/Asap3 antibody A10444 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
M. Bhatt
Verified customer
Asked: 2014-01-01
Answer
The anti-Uplc1/Asap3 antibody (A10444) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-01-01