Click image to see more details
Product Info Summary
| SKU: | PB9843 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-UPF3B/RENT3B Antibody Picoband®
SKU/Catalog Number
PB9843
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-UPF3B/RENT3B Antibody Picoband® catalog # PB9843. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-UPF3B/RENT3B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9843)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human UPF3B /RENT3B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9843 is reactive to UPF3B in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
58 kDa
Calculated molecular weight
57762 MW
Background of UPF3B
Regulator of nonsense transcripts 3B is a protein that in humans is encoded by the UPF3B gene. This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9843 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: human Hela whole cell, human 22RV1 whole cell, human U87 whole cell, human Jurkat whole cell, rat brain tissue
IHC: mouse testis tissue, rat testis tissue, human placenta tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of UPF3B/RENT3B using anti-UPF3B/RENT3B antibody (PB9843).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 22RV1 whole cell lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UPF3B/RENT3B antigen affinity purified polyclonal antibody (Catalog # PB9843) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UPF3B/RENT3B at approximately 58 kDa. The expected band size for UPF3B/RENT3B is at 58 kDa.
Click image to see more details
Figure 2. IHC analysis of UPF3B/RENT3B using anti-UPF3B/RENT3B antibody (PB9843).
UPF3B/RENT3B was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-UPF3B/RENT3B Antibody (PB9843) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of UPF3B/RENT3B using anti-UPF3B/RENT3B antibody (PB9843).
UPF3B/RENT3B was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-UPF3B/RENT3B Antibody (PB9843) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of UPF3B/RENT3B using anti-UPF3B/RENT3B antibody (PB9843).
UPF3B/RENT3B was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-UPF3B/RENT3B Antibody (PB9843) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For UPF3B (Source: Uniprot.org, NCBI)
Gene Name
UPF3B
Full Name
Regulator of nonsense transcripts 3B
Weight
57762 MW
Superfamily
RENT3 family
Alternative Names
Regulator of nonsense transcripts 3B;Nonsense mRNA reducing factor 3B;Up-frameshift suppressor 3 homolog B;hUpf3B;Up-frameshift suppressor 3 homolog on chromosome X;hUpf3p-X;UPF3B;RENT3B, UPF3X; UPF3B HUPF3B, MRX62, MRX82, MRXS14, RENT3BP1, UPF3BP2, UPF3BP3, UPF3X, Upf3p-X, UPF3B UPF3B regulator of nonsense mediated mRNA decay regulator of nonsense transcripts 3B|UPF3 regulator of nonsense transcripts homolog B|UPF3B pseudogene 1|UPF3B pseudogene 2|UPF3B pseudogene 3|mental retardation, X-linked 62|nonfunctional UPF3B regulator of nonsense mediated mRNA decay|nonsense mRNA reducing factor 3B|up-frameshift suppressor 3 homolog B|up-frameshift suppressor 3 homolog on chromosome X
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UPF3B, check out the UPF3B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UPF3B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-UPF3B/RENT3B Antibody Picoband® (PB9843)
Hello CJ!
PB9843 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Antisense suppression of the nonsense mediated decay factor Upf3b as a potential treatment for diseases caused by nonsense mutations
Species: Mouse
Huang L, Low A, Damle SS, Keenan MM, Kuntz S, Murray SF, Monia BP, Guo S. Genome Biol. 2018 Jan 15;19(1):4. doi: 10.1186/s13059-017-1386-9. Antisense suppression of the nonsense mediated decay factor Upf3b as a potential treatment for diseases cau...
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-UPF3B/RENT3B Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-UPF3B/RENT3B Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-UPF3B/RENT3B Antibody Picoband®
Question
Will anti-UPF3B/RENT3B antibody PB9843 work on primate WB with colon carcinoma?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
Our lab technicians have not tested anti-UPF3B/RENT3B antibody PB9843 on primate. You can run a BLAST between primate and the immunogen sequence of anti-UPF3B/RENT3B antibody PB9843 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate colon carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-13
Question
We are currently using anti-UPF3B/RENT3B antibody PB9843 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
The anti-UPF3B/RENT3B antibody (PB9843) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-11
Question
Would PB9843 anti-UPF3B/RENT3B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Zhao
Verified customer
Asked: 2018-08-09
Answer
It shows on the product datasheet, PB9843 anti-UPF3B/RENT3B antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-09
Question
Will anti-UPF3B/RENT3B antibody PB9843 work for WB with cervix carcinoma erythroleukemia?
C. Rodriguez
Verified customer
Asked: 2017-05-16
Answer
According to the expression profile of cervix carcinoma erythroleukemia, UPF3B is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-UPF3B/RENT3B antibody PB9843 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2017-05-16
Question
Do you have a BSA free version of anti-UPF3B/RENT3B antibody PB9843 available?
T. Krishna
Verified customer
Asked: 2014-09-05
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-UPF3B/RENT3B antibody PB9843 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-09-05
Question
Is a blocking peptide available for product anti-UPF3B/RENT3B antibody (PB9843)?
A. Krishna
Verified customer
Asked: 2014-07-17
Answer
We do provide the blocking peptide for product anti-UPF3B/RENT3B antibody (PB9843). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-07-17
Question
I am looking for to test anti-UPF3B/RENT3B antibody PB9843 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-UPF3B/RENT3B antibody PB9843 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Jha
Verified customer
Asked: 2014-03-25
Answer
The products we sell, including anti-UPF3B/RENT3B antibody PB9843, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-03-25
