Anti-UCMA Antibody

Product Name

Anti-UCMA Antibody

View all Ucma Antibodies

SKU/Catalog Number

A12617

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-UCMA Antibody (Catalog # A12617). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

UCMA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-UCMA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12617)

Host

Rabbit

Contents

UCMA Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

UCMA antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human UCMA. The immunogen is located within the last 50 amino acids of UCMA.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of N4BP1 are known to exist; this antibody will only detect the longest isoform.

Reactive Species

A12617 is reactive to UCMA in Human, Mouse

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

16563 MW

Background of Ucma

UCMA is a secreted cartilage-specific protein that was discovered in a screen for differentially expressed genes in retinoic acid-treated mouse chondrocytes. It was also identified in a human chondrocyte EST screen for candidate genes of skeletal dysplasias. UCMA expression is thought to parallel that of collagen II with its expression decreasing with maturation chrondrocytes mature. UCMA is processed by a furin-like protease into two fragments, an amino-terminal fragment and a carboxy-terminal fragment (UCMA-C). Application of recombinant UCMA-C to primary osteoblasts, mesenchymal stem cells, and MC3T3-E1 pre-osteoblasts interferes with their osteogenic differentiation, but does not affect expression of chondrocyte-specific genes or chondrocyte proliferation, suggesting that UCMA may be involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells. At least two isoforms of UCMA are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A12617 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

UCMA antibody can be used for detection of UCMA by Western blot at 2.5 - 5 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For UCMA (Source: Uniprot.org, NCBI)

Alternative Names

GRP, C10orf49, Unique cartilage matrix-associated protein, Gla-rich protein, Ucma-C Ucma|1110017I16Rik, AW121955, Grp|upper zone of growth plate and cartilage matrix associated|unique cartilage matrix-associated protein|Gla-rich protein|upper zone of growth plate and cartilage matrix associated protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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