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Product Info Summary
| SKU: | M02848 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Ubiquitin UBB Rabbit Monoclonal Antibody
SKU/Catalog Number
M02848
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Ubiquitin UBB Rabbit Monoclonal Antibody catalog # M02848. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ubiquitin UBB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02848)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IBI-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Ubiquitin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02848 is reactive to UBB in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
55 kDa
Calculated molecular weight
25762 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02848 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: HepG2 cell
IHC: human breast cancer tissue
ICC/IF: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of Ubiquitin expression in HepG2 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using Ubiquitin Antibody.
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Immunofluorescent analysis of Jurkat cells, using Ubiquitin Antibody.
Protein Target Info & Infographic
Gene/Protein Information For UBB (Source: Uniprot.org, NCBI)
Gene Name
UBB
Full Name
Polyubiquitin-B
Weight
25762 MW
Superfamily
ubiquitin family
Alternative Names
Polyubiquitin-B;Ubiquitin;UBB; UBB HEL-S-50 ubiquitin B polyubiquitin-B|epididymis secretory protein Li 50|polyubiquitin B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBB, check out the UBB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ubiquitin UBB Rabbit Monoclonal Antibody (M02848)
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No publications found for M02848
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Ubiquitin UBB Rabbit Monoclonal Antibody
Question
Will M02848 anti-Ubiquitin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
It shows on the product datasheet, M02848 anti-Ubiquitin Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-07
Question
Is a blocking peptide available for product anti-Ubiquitin Rabbit Monoclonal antibody (M02848)?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
We do provide the blocking peptide for product anti-Ubiquitin Rabbit Monoclonal antibody (M02848). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-09
Question
We were well pleased with the WB result of your anti-Ubiquitin Rabbit Monoclonal antibody. However we have been able to see positive staining in blood ubiquitin: cytoplasm. nucleus using this antibody. Is that expected? Could you tell me where is UBB supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
From what I have seen in literature, blood does express UBB. Generally UBB expresses in ubiquitin: cytoplasm. nucleus. Regarding which tissues have UBB expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3029682
Brain, Liver, and Lung, Pubmed ID: 15489334
Fetal brain cortex, Pubmed ID: 1128706
Boster Scientific Support
Answered: 2019-12-16
Question
Does anti-Ubiquitin Rabbit Monoclonal antibody M02848 work for IF with blood?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
According to the expression profile of blood, UBB is highly expressed in blood. So, it is likely that anti-Ubiquitin Rabbit Monoclonal antibody M02848 will work for IF with blood.
Boster Scientific Support
Answered: 2019-11-20
Question
See below the WB image, lot number and protocol we used for blood using anti-Ubiquitin Rabbit Monoclonal antibody M02848. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-02
Question
Do you have a BSA free version of anti-Ubiquitin Rabbit Monoclonal antibody M02848 available?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Ubiquitin Rabbit Monoclonal antibody M02848 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-17
Question
I was wanting to use your anti-Ubiquitin Rabbit Monoclonal antibody for IF for rat blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat blood identification?
Verified Customer
Verified customer
Asked: 2019-02-15
Answer
You can see on the product datasheet, M02848 anti-Ubiquitin Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-15
Question
We have been able to see staining in rat hypothalamus. Any tips? Is anti-Ubiquitin Rabbit Monoclonal antibody supposed to stain hypothalamus positively?
Verified Customer
Verified customer
Asked: 2018-12-27
Answer
Based on literature hypothalamus does express UBB. Based on Uniprot.org, UBB is expressed in hypothalamus, blood, brain, liver lung, fetal brain cortex, among other tissues. Regarding which tissues have UBB expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3029682
Brain, Liver, and Lung, Pubmed ID: 15489334
Fetal brain cortex, Pubmed ID: 1128706
Boster Scientific Support
Answered: 2018-12-27
Question
I see that the anti-Ubiquitin Rabbit Monoclonal antibody M02848 works with IF, what is the protocol used to produce the result images on the product page?
T. Baker
Verified customer
Asked: 2018-09-03
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-09-03
Question
I am looking for to test anti-Ubiquitin Rabbit Monoclonal antibody M02848 on rat blood for research purposes, then I may be interested in using anti-Ubiquitin Rabbit Monoclonal antibody M02848 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-07-27
Answer
The products we sell, including anti-Ubiquitin Rabbit Monoclonal antibody M02848, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-27
Question
Is this M02848 anti-Ubiquitin Rabbit Monoclonal antibody reactive to the isotypes of UBB?
Verified Customer
Verified customer
Asked: 2018-02-21
Answer
The immunogen of M02848 anti-Ubiquitin Rabbit Monoclonal antibody is A synthesized peptide derived from human Ubiquitin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-02-21
Question
We are currently using anti-Ubiquitin Rabbit Monoclonal antibody M02848 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-06
Answer
The anti-Ubiquitin Rabbit Monoclonal antibody (M02848) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-06
Question
you antibody using your anti-Ubiquitin Rabbit Monoclonal antibody for transcription-coupled nucleotide-excision repair studies. Has this antibody been tested with western blotting on hepg2 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-05-26
Answer
Thanks for your inquiry. This M02848 anti-Ubiquitin Rabbit Monoclonal antibody is tested on hepg2 cell lysate. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-05-26
Question
We purchased anti-Ubiquitin Rabbit Monoclonal antibody for IF on liver lung in the past. I am using rat, and We want to use the antibody for WB next. I am interested in examining liver lung as well as hypothalamus in our next experiment. Could give a recommendation on which antibody would work the best for WB?
P. Walker
Verified customer
Asked: 2016-07-01
Answer
I viewed the website and datasheets of our anti-Ubiquitin Rabbit Monoclonal antibody and it appears that M02848 has been validated on rat in both IF and WB. Thus M02848 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-07-01
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-Ubiquitin Rabbit Monoclonal antibody M02848. Let me know if you need anything else.
J. Krishna
Verified customer
Asked: 2014-12-19
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-12-19
Question
My question regarding product M02848, anti-Ubiquitin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
F. Johnson
Verified customer
Asked: 2014-10-24
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02848 anti-Ubiquitin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-10-24
