Anti-UBE2J1/Ubc6E Antibody

Product Name

Anti-UBE2J1/Ubc6E Antibody

View all UBE2J1 Antibodies

SKU/Catalog Number

A07059

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-UBE2J1/Ubc6E Antibody (Catalog # A07059). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-UBE2J1/Ubc6E Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07059)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near the amino terminus of the human Ube2j1 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07059 is reactive to UBE2J1 in Human

Reconstitution

Calculated molecular weight

35199 MW

Background of UBE2J1

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Ube2j1 and Ube2j2 are homologs of the yeast ubiquitin-conjugating enzyme UBC6, which catalyzes the covalent attachment of ubiquitin to other proteins. These proteins constitute a distinct family of ubiquitin-conjugating enzymes sharing a conserved non-canonical active site sequence and a C-terminal transmembrane domain. By analogy with yeast UBC6, Ube2j1 and Ube2j2 are localized to the endoplasmic reticulum and seem to function in the selective degradation of misfolded membrane proteins and in general mediation of the stress response.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A07059 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:1,000 - 1:5,000
IP: 1-2 µg
WB: 1:200 - 1:2,000

Validation Images & Assay Conditions

Gene/Protein Information For UBE2J1 (Source: Uniprot.org, NCBI)

Alternative Names

UBE2J1, E2 J1, Ubiquitin conjugating enzyme E2J1, Non-canonical ubiquitin-conjugating enzyme 1, Ube2j1, E2 ubiquitin-conjugating enzyme J1, NCUBE-1, Yeast ubiquitin-conjugating enzyme UBC6 homolog E, HsUBC6e UBE2J1 CGI-76, HSPC153, HSPC205, HSU93243, NCUBE-1, NCUBE1, UBC6, UBC6E, Ubc6p ubiquitin conjugating enzyme E2 J1 ubiquitin-conjugating enzyme E2 J1|E2 ubiquitin-conjugating enzyme J1|HSUBC6e|non-canonical ubiquitin-conjugating enzyme 1|ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast)|ubiquitin-conjugating enzyme E2, J1, U|yeast ubiquitin-conjugating enzyme UBC6 homolog E

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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