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Product Info Summary
| SKU: | M02295 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-UBE2I/Ubc9 Rabbit Monoclonal Antibody
View all UBE2I/Ubc9 Antibodies
SKU/Catalog Number
M02295
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-UBE2I/Ubc9 Rabbit Monoclonal Antibody catalog # M02295. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-UBE2I/Ubc9 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02295)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EDF-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human UBE2I
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02295 is reactive to UBE2I in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
38 kDa
Calculated molecular weight
18007 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02295 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
IHC: human kidney tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human kidney, using UBE2I Antibody.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Western blot analysis of UBE2I expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For UBE2I (Source: Uniprot.org, NCBI)
Gene Name
UBE2I
Full Name
SUMO-conjugating enzyme UBC9
Weight
18007 MW
Superfamily
ubiquitin-conjugating enzyme family
Alternative Names
SUMO-conjugating enzyme UBC9;6.3.2.-;SUMO-protein ligase;Ubiquitin carrier protein 9;Ubiquitin carrier protein I;Ubiquitin-conjugating enzyme E2 I;Ubiquitin-protein ligase I;p18;UBE2I;UBC9, UBCE9; UBE2I C358B7.1, P18, UBC9 ubiquitin conjugating enzyme E2 I SUMO-conjugating enzyme UBC9|RING-type E3 SUMO transferase UBC9|SUMO-1-protein ligase|SUMO-protein ligase|ubiquitin carrier protein 9|ubiquitin carrier protein I|ubiquitin conjugating enzyme 9|ubiquitin conjugating enzyme E2I|ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)|ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)|ubiquitin-conjugating enzyme UbcE2A|ubiquitin-like protein SUMO-1 conjugating enzyme|ubiquitin-protein ligase E2I|ubiquitin-protein ligase I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBE2I, check out the UBE2I Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBE2I: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-UBE2I/Ubc9 Rabbit Monoclonal Antibody (M02295)
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No publications found for M02295
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-UBE2I/Ubc9 Rabbit Monoclonal Antibody
Question
Does anti-UBE2I/Ubc9 Rabbit Monoclonal antibody M02295 work for IP with cervix carcinoma erythroleukemia?
C. Li
Verified customer
Asked: 2020-02-26
Answer
According to the expression profile of cervix carcinoma erythroleukemia, UBE2I is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-UBE2I/Ubc9 Rabbit Monoclonal antibody M02295 will work for IP with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2020-02-26
Question
I am interested in to test anti-UBE2I/Ubc9 Rabbit Monoclonal antibody M02295 on rat cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-UBE2I/Ubc9 Rabbit Monoclonal antibody M02295 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
The products we sell, including anti-UBE2I/Ubc9 Rabbit Monoclonal antibody M02295, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-29
Question
See below the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-UBE2I/Ubc9 Rabbit Monoclonal antibody M02295. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-23
Question
Will anti-UBE2I/Ubc9 Rabbit Monoclonal antibody M02295 work on horse IP with fetal brain?
S. Baker
Verified customer
Asked: 2019-04-01
Answer
Our lab technicians have not validated anti-UBE2I/Ubc9 Rabbit Monoclonal antibody M02295 on horse. You can run a BLAST between horse and the immunogen sequence of anti-UBE2I/Ubc9 Rabbit Monoclonal antibody M02295 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse fetal brain in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-01
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-UBE2I/Ubc9 Rabbit Monoclonal antibody M02295. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-06-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-12
Question
I was wanting to use your anti-UBE2I/Ubc9 Rabbit Monoclonal antibody for IP for rat cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2018-04-03
Answer
It shows on the product datasheet, M02295 anti-UBE2I/Ubc9 Rabbit Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-03
