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Product Info Summary
| SKU: | A08637 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-UBE1L UBA7 Antibody
View all ISG15 Activating Enzyme/UBE1L Antibodies
SKU/Catalog Number
A08637
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-UBE1L UBA7 Antibody (Catalog # A08637). Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-UBE1L UBA7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08637)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human UBE1L.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08637 is reactive to UBA7 in Human
Reconstitution
Calculated molecular weight
111694 MW
Background of ISG15 Activating Enzyme/UBE1L
Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Catalyzes the ISGylation of influenza A virus NS1 protein.
Sutisak Kitareewan, PNAS, Mar 2002; 99: 3806.
Keun Il Kim, Mol. Cell. Biol., Jan 2006; 26: 472 - 479.
Ian Pitha-Rowe, Cancer Res., Nov 2004; 64: 8109 - 8115.<
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08637 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from HeLa cells, using UBE1L antibody A08637.
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Western blot analysis of extracts from JK cells (Lane 2), using UBE1L antiobdy A08637. The lane on the left is treated with synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using UBE1L antibody A08637.
Protein Target Info & Infographic
Gene/Protein Information For UBA7 (Source: Uniprot.org, NCBI)
Gene Name
UBA7
Full Name
Ubiquitin-like modifier-activating enzyme 7
Weight
111694 MW
Superfamily
ubiquitin-activating E1 family
Alternative Names
Ubiquitin-like modifier-activating enzyme 7;Ubiquitin-activating enzyme 7;D8;Ubiquitin-activating enzyme E1 homolog;UBA7;UBE1L, UBE2; UBA7 D8, UBA1B, UBE1L, UBE2 ubiquitin like modifier activating enzyme 7 ubiquitin-like modifier-activating enzyme 7|UBA1, ubiquitin-activating enzyme E1 homolog B|UBA7, ubiquitin-activating enzyme E1|ubiquitin-activating enzyme 7|ubiquitin-activating enzyme E1 homolog|ubiquitin-activating enzyme E1-related protein|ubiquitin-activating enzyme-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBA7, check out the UBA7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBA7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-UBE1L UBA7 Antibody (A08637)
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No publications found for A08637
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-UBE1L UBA7 Antibody
Question
Does A08637 anti-UBE1L antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Li
Verified customer
Asked: 2020-05-08
Answer
You can see on the product datasheet, A08637 anti-UBE1L antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-08
Question
We are currently using anti-UBE1L antibody A08637 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
The anti-UBE1L antibody (A08637) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-05
Question
Please see the WB image, lot number and protocol we used for b-cell using anti-UBE1L antibody A08637. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-11
Question
I see that the anti-UBE1L antibody A08637 works with WB, what is the protocol used to produce the result images on the product page?
H. Wu
Verified customer
Asked: 2019-07-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-17
Question
Would anti-UBE1L antibody A08637 work on bovine WB with b-cell?
Verified Customer
Verified customer
Asked: 2017-06-28
Answer
Our lab technicians have not tested anti-UBE1L antibody A08637 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-UBE1L antibody A08637 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine b-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-28
Question
I have a question about product A08637, anti-UBE1L antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Edwards
Verified customer
Asked: 2013-01-02
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08637 anti-UBE1L antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-01-02
