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Product Info Summary
| SKU: | PB9904 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-E1 Ubiquitin Activating Enzyme/UBA1 Antibody Picoband®
View all Ubiquitin-activating Enzyme/UBE1 Antibodies
SKU/Catalog Number
PB9904
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-E1 Ubiquitin Activating Enzyme/UBA1 Antibody Picoband® catalog # PB9904. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-E1 Ubiquitin Activating Enzyme/UBA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9904)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human UBA1, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9904 is reactive to UBA1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
117 kDa
Calculated molecular weight
117849 MW
Background of Ubiquitin-activating Enzyme/UBE1
Ubiquitin-like modifier activating enzyme 1 (UBA1) is an enzyme which in humans is encoded by the UBA1 gene. The protein encoded by this gene catalyzes the first step in ubiquitin conjugation, or ubiquitination, to mark cellular proteins for degradation. Specifically, UBA1 catalyzes the ATP-dependent adenylation of ubiquitin (Ub), thereby forming a thioester bond between the two. It also continues to participate in subsequent steps of ubiquination as a Ub carrier. UBA1 is one of only two human ubiquitin-activating enzymes (E1), the other being UBA6, and thus is largely responsible for protein ubiquitination in humans. Through its central role in ubiquitination, UBA1 has been linked to cell cycle regulation, endocytosis, signal transduction, apoptosis, DNA damage repair, and transcriptional regulation. Additionally, UBE1 helps regulate the NEDD8 pathway, thus implicating it in protein folding, as well as mitigating the depletion of ubiquitin levels during stress.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9904 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat liver tissue, mouse liver tissuemouse testis tissueHELA whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of UBA1 using anti-UBA1 antibody (PB9904).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates.
Lane 3: mouse testis tissue lysates.
Lane 4: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBA1 antigen affinity purified polyclonal antibody (Catalog # PB9904) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBA1 at approximately 117 kDa. The expected band size for UBA1 is at 118 kDa.
Protein Target Info & Infographic
Gene/Protein Information For UBA1 (Source: Uniprot.org, NCBI)
Gene Name
UBA1
Full Name
Ubiquitin-like modifier-activating enzyme 1
Weight
117849 MW
Superfamily
ubiquitin-activating E1 family
Alternative Names
Ubiquitin-like modifier-activating enzyme 1;6.2.1.45 ;Protein A1S9;Ubiquitin-activating enzyme E1;UBA1;A1S9T, UBE1; UBA1 A1S9, A1S9T, A1ST, AMCX1, CFAP124, GXP1, POC20, SMAX2A, UBE1, UBE1X, VEXAS, UBA1 ubiquitin like modifier activating enzyme 1 ubiquitin-like modifier-activating enzyme 1|A1S9T and BN75 temperature sensitivity complementing|POC20 centriolar protein homolog|UBA1, ubiquitin-activating enzyme E1 homolog A|testicular secretory protein Li 63
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBA1, check out the UBA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-E1 Ubiquitin Activating Enzyme/UBA1 Antibody Picoband® (PB9904)
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1 Customer Q&As for Anti-E1 Ubiquitin Activating Enzyme/UBA1 Antibody Picoband®
Question
We are currently using anti-E1 Ubiquitin Activating Enzyme/UBA1 antibody PB9904 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
The anti-E1 Ubiquitin Activating Enzyme/UBA1 antibody (PB9904) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-05
