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- Table of Contents
Facts about Ubiquitin-like modifier-activating enzyme 1.
Essential for the formation of radiation- induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 in DNA damage sites (PubMed:22456334).
Human | |
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Gene Name: | UBA1 |
Uniprot: | P22314 |
Entrez: | 7317 |
Belongs to: |
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ubiquitin-activating E1 family |
A1S9T and BN75 temperature sensitivity complementing; A1S9T; A1ST; AMCX1; GXP1; MGC4781; POC20 centriolar protein homolog; POC20; Protein A1S9; SMAX2; UBA1; UBA1, ubiquitin-activating enzyme E1 homolog A; UBA1A; UBE1; UBE1A1S9; UBE1X; ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing); Ubiquitin-activating enzyme E1; Ubiquitinactivating Enzyme; Ubiquitin-activating Enzyme; ubiquitin-like modifier activating enzyme 1; ubiquitin-like modifier-activating enzyme 1
Mass (kDA):
117.849 kDA
Human | |
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Location: | Xp11.3 |
Sequence: | X; NC_000023.11 (47190847..47215128) |
Detected in erythrocytes (at protein level). Ubiquitous.
Cytoplasm. Mitochondrion. Nucleus.; [Isoform 1]: Nucleus.; [Isoform 2]: Cytoplasm.
If you're in search of an antibody against UBA1, Boster Bio has it! This antibody is designed to react with Human, UBA1 and has been tested for use in Flow Cytometry, IF and ICC. It can also be used for WB as well as Immunofluorescence ELISA. If you're not sure what kind of anti-UBA1 antibodies you need, you can take a look at Boster Bio's PICOKINE(tm) ELISA kit.
Researchers worldwide use Boster Bio's research primary and secondary anti-UBA1 antibodies for many applications, including immunohistochemistry, Western blot, ELISA, FC, and IHC. They have been thoroughly tested for use in a range of applications. They are designed for WB and ELISA tests and are in line with strict quality standards. Boster Bio also offers rabbit polyclonal antibody , as well as other products in its range of research antibodies.
The GATA1 antibody can also be used in immunohistochemistry, which can confirm localization and expression. Cells were fixed using 4% formaldehyde and left to dry for 30 minutes. The cells were then washed with PBST buffer (0.5g Tween-20 in PBS) and washed. The horse serum of 5% was used to block cells, and the primary antibodies were applied to the slides at 4 degC overnight. Antibodies against Sox2 and E1 were also added. After the primary antibody had been applied to the cell membrane, sections were rinsed with buffer PBST and probed with a second antibody against UBA1.
To understand the role played by UBA1 in neurogenesis, researchers have employed primal neuron cells of rats transfected with specific plasmids. These cells were treated for one week with puromycin, after that, ten colonies were picked for further analysis. To test Ube2 expression, Western blot analysis was performed using antibodies against HA. As an example, pCAG-3xHA left empty.
In addition to binding to the ALDh2A2 familyof proteins, UBE3A also interacts with the ALDh2A2 protein. These antibodies can also detect ALDh2A2-HA within 293-FT cells using UBE3A as a negative control. Boster Bio's primary as well as secondary antibodies for research using the UBA1 marker by Boster Bio allow researchers to detect immunofluorescence in SH-SY5Y cell lines.
Researchers often require a specific format and fluor for primary antibodies. Secondary reagents can be used in a variety of formats and are more versatile. Secondary antibodies can lead to the amplification of signals. Secondary antibodies usually have similar fluorophore to protein ratios as primary antibodies. Multiple secondary antibodies may bind to one primary antibody, creating an overall stronger signal. Additionally, researchers can utilize multiple primary antibodies in various applications.
The Boster Bio research primary and secondary antibodies for the UBA1 marker are available for human immunohistochemistry. The UBA1 marker is an relevant antigen to be identified in a variety of applications. Researchers can detect a variety of cancer with this marker which includes melanomas. There is a higher risk of cancer in people with a genetically-modified immune system This is why it is important to use a non-inferior-based primary antibody.
Boster Bio's research ELISA kits are a reliable and reproducible method of detecting the UBA1 marker. This kit has a picogram-level specificity and is suitable to detect biomarkers in many fields such as cancer, neurosciences, development biology, and many more. You can also get more information about Boster Bio's immunological reagents at Tebu-bio.
Boster Bio is a specialist in sensitive, high-specific antibodies. Boster Bio's ELISA kits can be used to test IHC or WB, as well as Flow. They have over 250 tissue samples. They are tested to determine whether they detect recombinant proteins. Boster Bio also offers validated ELISA kits for UBA1 which means they're sure to give you the results you're looking.
PicoKine(tm), ELISA kits, are built on Picokine(tm) technology, and reduce non-specific bound. They have sensitivity of the picogram and have been tested on a panel comprising more than 250 tissue samples. The ELISA kits are able to detect a wide range of proteins and antigens. They are compatible with all major ELISA systems. Boster Bio's ELISA kits are available through tebu-bio and other online retailers.
Boster Bio can be reached via their website or by calling them at. Boster Bio offers many products for the UBA1 marker. They offer ELISA kits, arrays and arrays. Boster Bio was one of the first Raybiotech labs to break into the European market and their website has thematic newsletters and lab services. The company has also expanded into other countries like Germany and France.
PMID: 1606621 by Ayusawa D., et al. Complementation by a cloned human ubiquitin-activating enzyme E1 of the S-phase-arrested mouse FM3A cell mutant with thermolabile E1.
PMID: 1986373 by Handley P.M., et al. Molecular cloning, sequence, and tissue distribution of the human ubiquitin-activating enzyme E1.