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Product Info Summary
| SKU: | M07252 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-UACA / Nucling (Nuclear Membrane Marker) Monoclonal Antibody
SKU/Catalog Number
M07252
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-UACA / Nucling (Nuclear Membrane Marker) Monoclonal Antibody (Catalog # M07252). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-UACA / Nucling (Nuclear Membrane Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07252)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: UACA/1222
Isotype
IgG1, kappa
Immunogen
Recombinant human UACA protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M07252 is reactive to UACA in Human, Mouse
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
162505 MW
Background of UACA
UACA (Uveal Autoantigen with Coiled-coil domains and Ankyrin repeats) is a 1,416 amino acid nuclear membrane protein. It was originally identified as an autoantigen in patients with panuveitis, a characteristic of Vogt-Koyanagi-Harada disease, and in patients with Graves' disease. UACA was also later identified as Nucling, an mRNA differentially expressed in F9 embryonal carcinoma cells during cardiac muscle differentiation. UACA appears to function as a pro-apoptotic protein that recruits the apaf-1-pro-caspase-9 complex for the induction of apoptosis to mediate the cell-death pathway.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07252 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Western Blot of A549 cell lysate using Anti-UACA / Nucling Monoclonal Antibody (UACA/1222)
Protein Target Info & Infographic
Gene/Protein Information For UACA (Source: Uniprot.org, NCBI)
Gene Name
UACA
Full Name
Uveal autoantigen with coiled-coil domains and ankyrin repeats
Weight
162505 MW
Alternative Names
Uveal autoantigen with coiled-coil domains and ankyrin repeats;UACA;KIAA1561; UACA NUCLING uveal auto with coiled-coil domains and ankyrin repeats uveal auto with coiled-coil domains and ankyrin repeats|nuclear membrane binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UACA, check out the UACA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UACA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-UACA / Nucling (Nuclear Membrane Marker) Monoclonal Antibody (M07252)
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No publications found for M07252
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-UACA / Nucling (Nuclear Membrane Marker) Monoclonal Antibody
Question
Does M07252 anti-UACA / Nucling (Nuclear Membrane Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
You can see on the product datasheet, M07252 anti-UACA / Nucling (Nuclear Membrane Marker) Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-01
Question
See attached the WB image, lot number and protocol we used for brain using anti-UACA / Nucling (Nuclear Membrane Marker) Monoclonal antibody M07252. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-10
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-UACA / Nucling (Nuclear Membrane Marker) Monoclonal antibody M07252. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-02
Question
Do you have a BSA free version of anti-UACA / Nucling (Nuclear Membrane Marker) Monoclonal antibody M07252 available?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-UACA / Nucling (Nuclear Membrane Marker) Monoclonal antibody M07252 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-31
Question
Is this M07252 anti-UACA / Nucling (Nuclear Membrane Marker) Monoclonal antibody reactive to the isotypes of UACA?
Verified Customer
Verified customer
Asked: 2017-09-19
Answer
The immunogen of M07252 anti-UACA / Nucling (Nuclear Membrane Marker) Monoclonal antibody is Recombinant human UACA protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-19
Question
We are currently using anti-UACA / Nucling (Nuclear Membrane Marker) Monoclonal antibody M07252 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on horse tissues as well?
R. Yang
Verified customer
Asked: 2016-12-19
Answer
The anti-UACA / Nucling (Nuclear Membrane Marker) Monoclonal antibody (M07252) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-12-19
