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Product Info Summary
| SKU: | A13510 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-TYW1 Antibody
SKU/Catalog Number
A13510
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TYW1 Antibody (Catalog # A13510). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Rat.
Storage & Handling
TYW1 antibody can be stored at 4 °C, stable for one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-TYW1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13510)
Host
Rabbit
Contents
TYW1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TYW1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human TYW1. The immunogen is located within amino acids 520 - 570 of TYW1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
TYW1 antibody is predicted to not cross-react with other TYW protein family members. At least three isoforms of TYW1 are known to exist; this antibody will only detect the two larger isoforms.
Reactive Species
A13510 is reactive to TYW1 in Human, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
83702 MW
Background of TYW1
TYW1, also known as TYW1A, RSAFD1 or YPL207W, is a 732 amino acid protein containing one flavodoxin-like domain that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW1 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW1 binds to one 4Fe-4S cluster and is located on human chromosome 7. Defects in some of the genes on chromosome 7 have been linked to Osteogenesis imperfecta, Williams-Beuren syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome, suggesting that TYW1 may play a role in these conditions
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13510 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TYW1 antibody can be used for detection of TYW1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TYW1 in human lung tissue lysate with TYW1 antibody at 1 μg/mL.
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Immunohistochemistry of TYW1 in human lung tissue with TYW1 antibody at 5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For TYW1 (Source: Uniprot.org, NCBI)
Gene Name
TYW1
Full Name
S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1
Weight
83702 MW
Superfamily
TYW1 family
Alternative Names
TYW1A, RSAFD1, YPL207W, S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase, Radical S-adenosyl methionine and flavodoxin domain-containing protein 1 TYW1 RSAFD1A, YPL207W, TYW1 tRNA-yW synthesizing protein 1 homolog S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1|radical S-adenosyl methionine and flavodoxin domain-containing protein 1|radical S-adenosyl methionine and flavodoxin domains 1|tRNA wybutosine-synthesizing protein 1 homolog|tRNA-yW synthesizing protein 1 homolog A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TYW1, check out the TYW1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TYW1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TYW1 Antibody (A13510)
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