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Product Info Summary
| SKU: | A00027-3 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-Tyrosine-protein kinase JAK2 JAK2 Antibody
SKU/Catalog Number
A00027-3
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Tyrosine-protein kinase JAK2 JAK2 Antibody (Catalog # A00027-3). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
JAK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Tyrosine-protein kinase JAK2 JAK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00027-3)
Host
Rabbit
Contents
JAK2 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
JAK2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human JAK2. The immunogen is located within the last 50 amino acids of JAK2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
JAK2 antibody is human, mouse and rat reactive. JAK2 antibody is predicted to not cross-react with other members of the JAK family of proteins.
Reactive Species
A00027-3 is reactive to JAK2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
130674 MW
Background of JAK2
JAK2 is a member of the JAK family of kinases that also include JAK1, JAK3, and TYK2 (1,2). JAK kinases are activated following tyrosine phosphorylation of cytokine receptors after ligand binding. JAK2 activation promotes the recruitment and phosphorylation of STAT3 and STAT5. These transcription factors then translocate to the nucleus where they bind specific DNA promoter sequences resulting in the transcription of genes that regulate cell proliferation, differentiation, and apoptosis (3). Mice with a disrupted JAK2 gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00027-3 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
JAK2 antibody can be used for detection of JAK2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of JAK2 in HeLa cell lysate with JAK2 antibody at 1 μg/ml.
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Immunohistochemistry of JAK2 in mouse kidney tissue with JAK2 antibody at 5 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For JAK2 (Source: Uniprot.org, NCBI)
Gene Name
JAK2
Full Name
Tyrosine-protein kinase JAK2
Weight
130674 MW
Superfamily
protein kinase superfamily
Alternative Names
Janus kinase 2, JTK10, THCYT3 JAK2 JTK10 Janus kinase 2 tyrosine-protein kinase JAK2|JAK-2|Janus kinase 2 (a protein tyrosine kinase)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on JAK2, check out the JAK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for JAK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tyrosine-protein kinase JAK2 JAK2 Antibody (A00027-3)
Hello CJ!
A00027-3 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang Q,Duan HX,Li RL,Sun JY,Liu J,Peng W,Wu CJ,Gao YX. Inducing Apoptosis and Suppressing Inflammatory Reactions in Synovial Fibroblasts are Two Important Ways for Guizhi-Shaoyao-Zhimu Decoction Against Rheumatoid Arthritis. J Inflamm Res.2021 Jan 26;14:
Species: Human
A00027-3 usage in article: APP:WB, SAMPLE:MH7A CELL, DILUTION:1:300
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