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Product Info Summary
| SKU: | M00913-5 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TYRO3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00913-5
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-TYRO3 Rabbit Monoclonal Antibody catalog # M00913-5. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TYRO3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00913-5)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
22T29
Isotype
IgG
Immunogen
A synthesized peptide derived from human TYRO3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00913-5 is reactive to TYRO3 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
120 kDa
Calculated molecular weight
49293 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00913-5 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:100-1:500
IP 1:50
Positive Control
WB: human MCF-7 whole cell, human U251 whole cell, human HepG2 whole cell, rat brain tissue, mouse brain tissue, mouse lung tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TYRO3 using anti-TYRO3 antibody (M00913-5).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TYRO3 antigen affinity purified monoclonal antibody (Catalog # M00913-5) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TYRO3 at approximately 120 kDa. The expected band size for TYRO3 is at 97 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TYRO3 (Source: Uniprot.org, NCBI)
Gene Name
TYRO3
Full Name
Tyrosine-protein kinase receptor TYRO3
Weight
49293 MW
Superfamily
protein kinase superfamily
Alternative Names
D (1A) dopamine receptor;Dopamine D1 receptor;DRD1; TYRO3 BYK, Dtk, Etk-2, RSE, Rek, Sky, Tif TYRO3 protein tyrosine kinase tyrosine-protein kinase receptor TYRO3|tyrosine-protein kinase DTK|tyrosine-protein kinase RSE|tyrosine-protein kinase SKY|tyrosine-protein kinase TIF|tyrosine-protein kinase byk
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TYRO3, check out the TYRO3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TYRO3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TYRO3 Rabbit Monoclonal Antibody (M00913-5)
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