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Product Info Summary
| SKU: | PA1903 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-TXNRD2 Antibody Picoband®
SKU/Catalog Number
PA1903
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TXNRD2 Antibody catalog # PA1903. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TXNRD2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1903)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TXNRD2, different from the related rat sequence by three amino acids, and from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1903 is reactive to TXNRD2 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
110 kDa
Calculated molecular weight
56507 MW
Background of TXNRD2
TXNRD2 (Thioredoxin reductase 2), also known as TRXR2, TR3, SELZ, or TR-BETA, Thioredoxin reductases, are selenocysteine (sec)-containing flavoenzymes that maintain thioredoxins, small proteins that catalyze redox reactions, in the reduced state using the reducing power of NADPH. By STS analysis and genomic sequence analysis, respectively, Miranda-Vizuete et al. (1999) and Sun et al. (1999) mapped the TXNRD2 gene to chromosome 22q11.2. Miranda-Vizuete et al. (1999) mapped the mouse gene to chromosome 16. Gasdaska et al. (1999) showed that TXNRD2 was a thioredoxin reductase that could directly reduce proteins such as insulin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1903 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: Rat Kidney Tissue, Rat Ovary Tissue, Rat Liver Tissue, SMMC Cell
IHC: Human Lung Cancer Tissue, rat intestine tissue
Validation Images & Assay Conditions
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Anti-TXNRD2 antibody, PA1903, Western blotting
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Ovary Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: SMMC Cell Lysate
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Anti-TXNRD2 antibody, PA1903, IHC(P)
IHC(P): Human Lung Cancer Tissue
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Figure 3. IHC analysis of TXNRD2 using anti-TXNRD2 antibody (PA1903).
TXNRD2 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TXNRD2 Antibody (PA1903) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For TXNRD2 (Source: Uniprot.org, NCBI)
Gene Name
TXNRD2
Full Name
Thioredoxin reductase 2, mitochondrial
Weight
56507 MW
Superfamily
class-I pyridine nucleotide-disulfide oxidoreductase family
Alternative Names
Thioredoxin reductase 2, mitochondrial;1.8.1.9;Selenoprotein Z;SelZ;TR-beta;Thioredoxin reductase TR3;TXNRD2;KIAA1652, TRXR2; TXNRD2 GCCD5, SELZ, TR, TR-BETA, TR3, TRXR2 thioredoxin reductase 2 thioredoxin reductase 2, mitochondrial|selenoprotein Z|thioredoxin reductase 3|thioredoxin reductase TR3|thioredoxin reductase beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TXNRD2, check out the TXNRD2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TXNRD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TXNRD2 Antibody Picoband® (PA1903)
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Customer Q&As
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3 Customer Q&As for Anti-TXNRD2 Antibody Picoband®
Question
Does anti-TXNRD2 antibody PA1903 work on feline IHC with adrenal gland testis?
H. Baker
Verified customer
Asked: 2016-09-08
Answer
Our lab technicians have not tested anti-TXNRD2 antibody PA1903 on feline. You can run a BLAST between feline and the immunogen sequence of anti-TXNRD2 antibody PA1903 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline adrenal gland testis in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-09-08
Question
I was wanting to use your anti-TXNRD2 antibody for WB for human fetal heart placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human fetal heart placenta identification?
L. Mitchell
Verified customer
Asked: 2016-03-22
Answer
It shows on the product datasheet, PA1903 anti-TXNRD2 antibody has been tested for IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human fetal heart placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-03-22
Question
We are currently using anti-TXNRD2 antibody PA1903 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on dog tissues as well?
C. Edwards
Verified customer
Asked: 2013-06-13
Answer
The anti-TXNRD2 antibody (PA1903) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-13
