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Product Info Summary
| SKU: | A09772-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-TXNDC17 Antibody Picoband®
View all Thioredoxin-like 5/TRP14/TXNDC17 Antibodies
SKU/Catalog Number
A09772-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-TXNDC17 Antibody Picoband® catalog # A09772-1. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TXNDC17 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09772-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TXNDC17 recombinant protein (Position: M1-D123).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09772-1 is reactive to TXNDC17 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
14 kDa
Calculated molecular weight
67169 MW
Background of Thioredoxin-like 5/TRP14/TXNDC17
Disulfide reductase. May participate in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyze dithiol-disulfide exchange reactions. Modulates TNF-alpha signaling and NF-kappa-B activation. Has peroxidase activity and may contribute to the elimination of cellular hydrogen peroxide.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09772-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Jurkat whole cell, rat kidney tissue, mouse kidney tissue
FCM: JK cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TXNDC17 using anti-TXNDC17 antibody (A09772-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXNDC17 antigen affinity purified polyclonal antibody (Catalog # A09772-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TXNDC17 at approximately 14 kDa. The expected band size for TXNDC17 is at 14 kDa.
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Figure 2. Flow Cytometry analysis of JK cells using anti-TXNDC17 antibody (A09772-1).
Overlay histogram showing JK cells stained with A09772-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TXNDC17 Antibody (A09772-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For TXNDC17 (Source: Uniprot.org, NCBI)
Gene Name
TXNDC17
Full Name
Thioredoxin domain-containing protein 17
Weight
67169 MW
Superfamily
thioredoxin family
Alternative Names
ATP synthase F (0) complex subunit C1,2,3, mitochondrial; ATP synthase lipid-binding protein; ATP synthase membrane subunit c locus 1,2,3; ATP synthase proteolipid P1; ATP synthase proton-transporting mitochondrial F (0) complex subunit C1,2,3; ATPase protein 9; ATPase subunit c; ATP5MC1,2,3; ATP5G1,2,3 Txndc17|4831443O22Rik, D11Ertd672, D11Ertd672e, TRP, TRP14, Txn, Txnl5|thioredoxin domain containing 17|thioredoxin domain-containing protein 17|14 kDa thioredoxin-related protein|protein 42-9-9|putative 42-9-9 protein|thioredoxin-like 5|thioredoxin-like protein 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TXNDC17, check out the TXNDC17 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TXNDC17: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TXNDC17 Antibody Picoband® (A09772-1)
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