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Product Info Summary
| SKU: | M00540 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P |
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Product info
Product Name
Anti-TTN Antibody (N-term)
SKU/Catalog Number
M00540
Size
50 µl
Description
Boster Bio Anti-TTN Antibody (N-term) (Catalog # M00540). Tested in IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-TTN Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00540)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This TTN antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 7169-7203 amino acids from the N-terminal region of human TTN.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00540 is reactive to TTN in Human
Reconstitution
Calculated molecular weight
3816030 Da
Background of Titin
Key component in the assembly and functioning of vertebrate striated muscles. By providing connections at the level of individual microfilaments, it contributes to the fine balance of forces between the two halves of the sarcomere. The size and extensibility of the cross-links are the main determinants of sarcomere extensibility properties of muscle. In non-muscle cells, seems to play a role in chromosome condensation and chromosome segregation during mitosis. Might link the lamina network to chromatin or nuclear actin, or both during interphase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00540 is guaranteed for IHC-P Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC-P: 1:500
Validation Images & Assay Conditions
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Immunohistochemical analysis of TITIN in Human skeletal muscle tissue sections (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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Immunohistochemical analysis of TITIN in Human heart tissue sections(IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For TTN (Source: Uniprot.org, NCBI)
Gene Name
TTN
Full Name
Titin
Weight
3816030 Da
Superfamily
protein kinase superfamily
Alternative Names
Titin, 2.7.11.1, Connectin, Rhabdomyosarcoma antigen MU-RMS-40.14, TTN TTN CMD1G, CMH9, CMPD4, EOMFC, HMERF, LGMD2J, LGMDR10, MYLK5, SALMY, TMD titin titin|connectin|rhabdomyosarcoma MU-RMS-40.14
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TTN, check out the TTN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TTN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TTN Antibody (N-term) (M00540)
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