Anti-TTF-1 / NKX2.1 (Thyroid & Lung Epithelial Marker) Monoclonal Antibody

Product Name

Anti-TTF-1 / NKX2.1 (Thyroid & Lung Epithelial Marker) Monoclonal Antibody

View all TTF-1 / NKX2-1 Antibodies

SKU/Catalog Number

M01322

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-TTF-1 / NKX2.1 (Thyroid & Lung Epithelial Marker) Monoclonal Antibody (Catalog # M01322). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-TTF-1 / NKX2.1 (Thyroid & Lung Epithelial Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01322)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM150

Isotype

IgG1, kappa

Immunogen

Rat full length TTF-1 recombinant protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01322 is reactive to NKX2-1 in Human, Mouse, Rat

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

38596 MW

Background of TTF-1 / NKX2-1

Recognizes a protein of 40kDa, identified as Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates.Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01322 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For NKX2-1 (Source: Uniprot.org, NCBI)

Alternative Names

Homeobox protein Nkx-2.1;Homeobox protein NK-2 homolog A;Thyroid nuclear factor 1;Thyroid transcription factor 1;TTF-1;Thyroid-specific enhancer-binding protein;T/EBP;NKX2-1 ;NKX2A, TITF1, TTF1; NKX2-1 BCH, BHC, NK-2, NKX2.1, NKX2A, NMTC1, T/EBP, TEBP, TITF1, TTF-1, TTF1 NK2 homeobox 1 homeobox protein Nkx-2.1|NK-2 homolog A|homeobox protein NK-2 homolog A|thyroid nuclear factor 1|thyroid transcription factor 1|thyroid-specific enhancer-binding protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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