Product Info Summary
SKU: | A07022-2 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-TSEN54 Antibody Picoband™
SKU/Catalog Number
A07022-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-TSEN54 Antibody Picoband™ catalog # A07022-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TSEN54 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07022-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TSEN54 recombinant protein (Position: D147-H507).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07022-2 is reactive to Tsen54 in Human
Applications
A07022-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
59 kDa
Calculated molecular weight
Background of Tsen54
This gene encodes a subunit of the tRNA splicing endonuclease complex, which catalyzes the removal of introns from precursor tRNAs. The complex is also implicated in pre-mRNA 3-prime end processing. Mutations in this gene result in pontocerebellar hypoplasia type 2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 µg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
![a07022 2 tsen54 primary antibodies wb testing 1 a07022 2 tsen54 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a07022-2-tsen54-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of TSEN54 using anti-TSEN54 antibody (A07022-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human MOLT-4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSEN54 antigen affinity purified polyclonal antibody (Catalog # A07022-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSEN54 at approximately 59 kDa. The expected band size for TSEN54 is at 59,20 kDa.
![a07022 2 tsen54 primary antibodies ihc testing 2 a07022 2 tsen54 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a07022-2-tsen54-primary-antibodies-ihc-testing-2.jpg)
Click image to see more details
Figure 2. IHC analysis of TSEN54 using anti-TSEN54 antibody (A07022-2).
TSEN54 was detected in a paraffin-embedded section of human Colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TSEN54 Antibody (A07022-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a07022 2 tsen54 primary antibodies if testing 3 a07022 2 tsen54 primary antibodies if testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a07022-2-tsen54-primary-antibodies-if-testing-3.jpg)
Click image to see more details
Figure 3. IF analysis of TSEN54 and Tubulin beta using anti-TSEN54 antibody (A07022-2) and anti-Tubulin beta antibody (M05613-4).
TSEN54 and Tubulin beta was detected in an immunocytochemical section of PC3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TSEN54 Antibody (A07022-2) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a07022 2 tsen54 primary antibodies fcm testing 4 a07022 2 tsen54 primary antibodies fcm testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a07022-2-tsen54-primary-antibodies-fcm-testing-4.png)
Click image to see more details
Figure 4. Flow Cytometry analysis of Hela cells using anti-TSEN54 antibody (A07022-2).
Overlay histogram showing Hela cells stained with A07022-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TSEN54 Antibody (A07022-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Tsen54 (Source: Uniprot.org, NCBI)
Gene Name
Tsen54
Full Name
tRNA-splicing endonuclease subunit Sen54
Weight
Superfamily
SEN54 family
Alternative Names
tRNA-splicing endonuclease subunit Sen54
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tsen54, check out the Tsen54 Infographic
![Tsen54 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tsen54: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TSEN54 Antibody Picoband™ (A07022-2)
Hello CJ!
No publications found for A07022-2
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-TSEN54 Antibody Picoband™?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-TSEN54 Antibody Picoband™
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question