Anti-TSEN54 Antibody

Product Name

Anti-TSEN54 Antibody

View all Tsen54 Antibodies

SKU/Catalog Number

A07022

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-TSEN54 Antibody (Catalog # A07022). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TSEN54 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07022)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human TSEN54.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07022 is reactive to Tsen54 in Human, Mouse

Applications

A07022 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

58819 MW

Background of Tsen54

Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events.

Zody M.C., Nature 440:1045-1049(2006).
Paushkin S.V., Cell 117:311-321(2004).
Ota T., Nat. Genet. 36:40-45(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

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Western blot analysis of extracts from 3T3 cells and Jurkat cells, using TSEN54 antibody A07022.

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using TSEN54 antibody A07022.

Gene/Protein Information For Tsen54 (Source: Uniprot.org, NCBI)

Alternative Names

tRNA-splicing endonuclease subunit Sen54

For more info on Tsen54, check out the Tsen54 Infographic

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In this infographic, you will see the following information for Tsen54: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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