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Product Info Summary
| SKU: | P01626 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-Tryptophan Hydroxylase (Ser260) Antibody
View all Tryptophan Hydroxylase 1/TPH-1 Antibodies
SKU/Catalog Number
P01626
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-Tryptophan Hydroxylase (Ser260) Antibody (Catalog # P01626). Tested in WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-Tryptophan Hydroxylase (Ser260) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01626)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser260 of rat tryptophan hydroxylase (TPH), conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P01626 is reactive to TPH1 in Human, Rat
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
50985 MW
Background of Tryptophan Hydroxylase 1/TPH-1
Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser-260 which lies within the regulatory domain of TPH (Jiang et al., 2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01626 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
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Western blot of rat brainstem lysate showing specific immunolabeling of the ~55 kDa TPH protein phosphorylated at Ser260 in lane one (-). Phosphospecificity is shown in the second lane (P) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (NP), shown in the third lane.
Protein Target Info & Infographic
Gene/Protein Information For TPH1 (Source: Uniprot.org, NCBI)
Gene Name
TPH1
Full Name
Tryptophan 5-hydroxylase 1
Weight
50985 MW
Superfamily
biopterin-dependent aromatic amino acid hydroxylase family
Alternative Names
Indoleacetic acid 5 hydroxylase antibody, L tryptophan hydroxylase antibody, MGC119994 antibody, TPH 1 antibody, TPH antibody, TPH1 antibody, TPH1_HUMAN antibody, TPRH antibody, TRPH antibody, Tryptophan 5 hydroxylase 1 antibody, Tryptophan 5 monooxygenase 1 antibody, Tryptophan 5 monooxygenase antibody, Tryptophan 5-hydroxylase 1 antibody, Tryptophan 5-monooxygenase 1 antibody, Tryptophan hydroxylase 1 antibody TPH1 TPRH, TRPH tryptophan hydroxylase 1 tryptophan 5-hydroxylase 1|L-tryptophan hydroxylase|indoleacetic acid-5-hydroxylase|tryptophan 5-monooxygenase 1|tryptophan hydroxylase (tryptophan 5-monooxygenase)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TPH1, check out the TPH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TPH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Tryptophan Hydroxylase (Ser260) Antibody (P01626)
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