Product Info Summary
SKU: | P01626-1 |
---|---|
Size: | 100 µl |
Reactive Species: | Mouse, Rabbit |
Host: | Rabbit |
Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Phospho-Tryptophan Hydroxylase (Ser58) Antibody
View all Tryptophan Hydroxylase 1/TPH-1 Antibodies
SKU/Catalog Number
P01626-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-Tryptophan Hydroxylase (Ser58) Antibody (Catalog # P01626-1). Tested in WB applications. This antibody reacts with Mouse, Rabbit.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-Tryptophan Hydroxylase (Ser58) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01626-1)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 of rat tryptophan hydroxylase 1 (TPH1), conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P01626-1 is reactive to TPH1 in Mouse, Rabbit
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
50985 MW
Background of Tryptophan Hydroxylase 1/TPH-1
Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996) Both PKA and CaM K II phosphorylate Ser-58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01626-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
Click image to see more details
Western blot of recombinant tryptophan hydroxylase incubated in the absence (-) and presence (+) of cAMP-dependent protein kinase showing specific immunolabeling of the ~53 kDa tryptophan hydroxylase protein phosphorylated at Ser58.
Protein Target Info & Infographic
Gene/Protein Information For TPH1 (Source: Uniprot.org, NCBI)
Gene Name
TPH1
Full Name
Tryptophan 5-hydroxylase 1
Weight
50985 MW
Superfamily
biopterin-dependent aromatic amino acid hydroxylase family
Alternative Names
Indoleacetic acid 5 hydroxylase antibody, L tryptophan hydroxylase antibody, MGC119994 antibody, TPH 1 antibody, TPH antibody, TPH1 antibody, TPH1_HUMAN antibody, TPRH antibody, TRPH antibody, Tryptophan 5 hydroxylase 1 antibody, Tryptophan 5 monooxygenase 1 antibody, Tryptophan 5 monooxygenase antibody, Tryptophan 5-hydroxylase 1 antibody, Tryptophan 5-monooxygenase 1 antibody, Tryptophan hydroxylase 1 antibody TPH1 TPRH, TRPH tryptophan hydroxylase 1 tryptophan 5-hydroxylase 1|L-tryptophan hydroxylase|indoleacetic acid-5-hydroxylase|tryptophan 5-monooxygenase 1|tryptophan hydroxylase (tryptophan 5-monooxygenase)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TPH1, check out the TPH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TPH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Tryptophan Hydroxylase (Ser58) Antibody (P01626-1)
Hello CJ!
No publications found for P01626-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Phospho-Tryptophan Hydroxylase (Ser58) Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Phospho-Tryptophan Hydroxylase (Ser58) Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-Phospho-Tryptophan Hydroxylase (Ser58) Antibody
Question
See attached the WB image, lot number and protocol we used for buccal mucosa cell using anti-Phospho-Tryptophan Hydroxylase (Ser58) antibody P01626-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-07
Question
I was wanting to use your anti-Phospho-Tryptophan Hydroxylase (Ser58) antibody for WB for rabbit buccal mucosa cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rabbit buccal mucosa cell identification?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
You can see on the product datasheet, P01626-1 anti-Phospho-Tryptophan Hydroxylase (Ser58) antibody has been tested for WB on rabbit tissues. We have an innovator award program that if you test this antibody and show it works in rabbit buccal mucosa cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-02
Question
Do you have a BSA free version of anti-Phospho-Tryptophan Hydroxylase (Ser58) antibody P01626-1 available?
V. Patel
Verified customer
Asked: 2019-10-02
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-Tryptophan Hydroxylase (Ser58) antibody P01626-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-02
Question
I am interested in to test anti-Phospho-Tryptophan Hydroxylase (Ser58) antibody P01626-1 on rabbit buccal mucosa cell for research purposes, then I may be interested in using anti-Phospho-Tryptophan Hydroxylase (Ser58) antibody P01626-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
The products we sell, including anti-Phospho-Tryptophan Hydroxylase (Ser58) antibody P01626-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-05
Question
Would P01626-1 anti-Phospho-Tryptophan Hydroxylase (Ser58) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-11-07
Answer
You can see on the product datasheet, P01626-1 anti-Phospho-Tryptophan Hydroxylase (Ser58) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-07