Anti-Phospho-Tryptophan Hydroxylase (Ser58) Antibody

Tryptophan Hydroxylase 1/TPH-1 antibody

Boster Bio Anti-Phospho-Tryptophan Hydroxylase (Ser58) Antibody (Catalog # P01626-1). Tested in WB applications. This antibody reacts with Mouse, Rabbit.

Product Info Summary

SKU: P01626-1
Size: 100 µl
Reactive Species: Mouse, Rabbit
Host: Rabbit
Application: WB

Product Name

Anti-Phospho-Tryptophan Hydroxylase (Ser58) Antibody

View all Tryptophan Hydroxylase 1/TPH-1 Antibodies

SKU/Catalog Number

P01626-1

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-Tryptophan Hydroxylase (Ser58) Antibody (Catalog # P01626-1). Tested in WB applications. This antibody reacts with Mouse, Rabbit.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-Tryptophan Hydroxylase (Ser58) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01626-1)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 of rat tryptophan hydroxylase 1 (TPH1), conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P01626-1 is reactive to TPH1 in Mouse, Rabbit

Reconstitution

Observed Molecular Weight

52 kDa

Calculated molecular weight

50985 MW

Background of Tryptophan Hydroxylase 1/TPH-1

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996) Both PKA and CaM K II phosphorylate Ser-58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

P01626-1 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

Gene/Protein Information For TPH1 (Source: Uniprot.org, NCBI)

Gene Name

TPH1

Full Name

Tryptophan 5-hydroxylase 1

Weight

50985 MW

Superfamily

biopterin-dependent aromatic amino acid hydroxylase family

Alternative Names

Indoleacetic acid 5 hydroxylase antibody, L tryptophan hydroxylase antibody, MGC119994 antibody, TPH 1 antibody, TPH antibody, TPH1 antibody, TPH1_HUMAN antibody, TPRH antibody, TRPH antibody, Tryptophan 5 hydroxylase 1 antibody, Tryptophan 5 monooxygenase 1 antibody, Tryptophan 5 monooxygenase antibody, Tryptophan 5-hydroxylase 1 antibody, Tryptophan 5-monooxygenase 1 antibody, Tryptophan hydroxylase 1 antibody TPH1 TPRH, TRPH tryptophan hydroxylase 1 tryptophan 5-hydroxylase 1|L-tryptophan hydroxylase|indoleacetic acid-5-hydroxylase|tryptophan 5-monooxygenase 1|tryptophan hydroxylase (tryptophan 5-monooxygenase)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TPH1, check out the TPH1 Infographic

TPH1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TPH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Phospho-Tryptophan Hydroxylase (Ser58) Antibody

Question

See attached the WB image, lot number and protocol we used for buccal mucosa cell using anti-Phospho-Tryptophan Hydroxylase (Ser58) antibody P01626-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-02-07

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-07

Question

I was wanting to use your anti-Phospho-Tryptophan Hydroxylase (Ser58) antibody for WB for rabbit buccal mucosa cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rabbit buccal mucosa cell identification?

Verified Customer

Verified customer

Asked: 2020-01-02

Answer

You can see on the product datasheet, P01626-1 anti-Phospho-Tryptophan Hydroxylase (Ser58) antibody has been tested for WB on rabbit tissues. We have an innovator award program that if you test this antibody and show it works in rabbit buccal mucosa cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-02

Question

Do you have a BSA free version of anti-Phospho-Tryptophan Hydroxylase (Ser58) antibody P01626-1 available?

V. Patel

Verified customer

Asked: 2019-10-02

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-Tryptophan Hydroxylase (Ser58) antibody P01626-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-10-02

Question

I am interested in to test anti-Phospho-Tryptophan Hydroxylase (Ser58) antibody P01626-1 on rabbit buccal mucosa cell for research purposes, then I may be interested in using anti-Phospho-Tryptophan Hydroxylase (Ser58) antibody P01626-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-09-05

Answer

The products we sell, including anti-Phospho-Tryptophan Hydroxylase (Ser58) antibody P01626-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-09-05

Question

Would P01626-1 anti-Phospho-Tryptophan Hydroxylase (Ser58) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-11-07

Answer

You can see on the product datasheet, P01626-1 anti-Phospho-Tryptophan Hydroxylase (Ser58) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-11-07

Order DetailsPrice
P01626-1

100uL

$481

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P01626-1
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$481.00

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