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Product Info Summary
| SKU: | A00128-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-TRPV1 Antibody Picoband®
View all Vanilloid R1/TRPV1 Antibodies
SKU/Catalog Number
A00128-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRPV1 Antibody Picoband® catalog # A00128-4. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TRPV1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00128-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TRPV1 recombinant protein (Position: E107-A800).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00128-4 is reactive to TRPV1 in Human, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
95 kDa
Calculated molecular weight
94.956kDa
Background of Vanilloid R1/TRPV1
The transient receptor potential cation channel, subfamily V, member 1(TRPV1), also known as the capsaicin receptor is a protein which in humans is encoded by the TRPV1 gene. TRPV1(also called Vanilloid receptor type 1) is a ligand-gated nonselective cation channel that is considered to be an important integrator of various pain stimuli such as endogenous lipids, capsaicin, heat, and low pH. In addition to expression in primary afferents, TRPV1 is also expressed in the CNS. Cui M et al.(2006) demonstrate that TRPV1 receptors in the CNS plays an important role in pain mediated by central sensitization. And the significant CNS penetration is necessary for a TRPV1 antagonist to produce broad-spectrum analgesia. And TRPV1 also participates in normal bladder function and is essential for normal mechanically evoked purinergic signaling by the urothelium.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00128-4 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human HepG2 whole cell, rat liver tissue, rat PC-12 whole cell
ICC/IF: T-47D cell
FCM: U87 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TRPV1 using anti-TRPV1 antibody (A00128-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPV1 antigen affinity purified polyclonal antibody (Catalog # A00128-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPV1 at approximately 95 kDa. The expected band size for TRPV1 is at 95 kDa.
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Figure 2. IF analysis of TRPV1 using anti-TRPV1 antibody (A00128-4).
TRPV1 was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TRPV1 Antibody (A00128-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of U87 cells using anti-TRPV1 antibody (A00128-4).
Overlay histogram showing U87 cells stained with A00128-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPV1 Antibody (A00128-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For TRPV1 (Source: Uniprot.org, NCBI)
Gene Name
TRPV1
Full Name
Transient receptor potential cation channel subfamily V member 1
Weight
94.956kDa
Superfamily
transient receptor (TC 1.A.4) family
Alternative Names
Proliferating cell nuclear antigen; PCNA; Cyclin; PCNA TRPV1 VR1 transient receptor potential cation channel subfamily V member 1 transient receptor potential cation channel subfamily V member 1|OTRPC1|capsaicin receptor|osm-9-like TRP channel 1|transient receptor potential vanilloid 1a|transient receptor potential vanilloid 1b|vanilloid receptor subtype 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRPV1, check out the TRPV1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRPV1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRPV1 Antibody Picoband® (A00128-4)
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