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Product Info Summary
| SKU: | A00122-1 |
|---|---|
| Size: | 100µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IP, IF, WB |
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Product info
Product Name
Anti-PARP-1 Antibody
SKU/Catalog Number
A00122-1
Size
100µl
Form
Liquid. Neat serum containing 0.1% sodium azide.
Description
Boster Bio Anti-PARP-1 Antibody catalog # A00122-1. Tested in ELISA, IP, IF, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
+4°C
Cite This Product
Anti-PARP-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00122-1)
Host
Rabbit
Contents
Liquid. Neat serum containing 0.1% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant human PARP-1 (poly (ADP-ribose) polymerase-1) (aa 1-1014) .
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00122-1 is reactive to PARP1 in Human, Mouse
Reconstitution
Calculated molecular weight
113084 MW
Background of PARP1
Participates in DNA repair and in chromosomal DNA replication.
Kesti T., J. Biol. Chem. 268:10238-10245(1993).
Syvaeoja J.E.;Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
Asahara H., Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00122-1 is guaranteed for ELISA, IP, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry (1:4,000)
Immunoprecipitation (1:400)
Western Blot (1:4,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PARP1 using anti-PARP1 antibody (A00122-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP1 antigen affinity purified polyclonal antibody (Catalog # A00122-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for PARP1.
Protein Target Info & Infographic
Gene/Protein Information For PARP1 (Source: Uniprot.org, NCBI)
Gene Name
PARP1
Full Name
Poly [ADP-ribose] polymerase 1
Weight
113084 MW
Alternative Names
Poly [ADP-ribose] polymerase 1;PARP-1;2.4.2.30 PARP1 ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PPOL, pADPRT-1 poly(ADP-ribose) polymerase 1 poly [ADP-ribose] polymerase 1|ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)|ADP-ribosyltransferase NAD(+)|ADP-ribosyltransferase diphtheria toxin-like 1|DNA ADP-ribosyltransferase PARP1|NAD(+) ADP-ribosyltransferase 1|poly (ADP-ribose) polymerase family, member 1|poly(ADP-ribose) synthetase|poly(ADP-ribosyl)transferase|poly[ADP-ribose] synthase 1|protein poly-ADP-ribosyltransferase PARP1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PARP1, check out the PARP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PARP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PARP-1 Antibody (A00122-1)
Hello CJ!
A00122-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wang P,Wang C,Liu C. Antitumor effects of dioscin in A431 cells via adjusting ATM/p53-mediated cell apoptosis, DNA damage and migration. Oncol Lett. 2021 Jan;21(1):59.doi:10.3892/ol.2020.12321.Epub 2020 Nov 19.PMID:33281970;PMCID:PMC7709553.
Species: Human
A00122-1 usage in article: APP:WB, SAMPLE:A431 CELLS, DILUTION:NA
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-PARP-1 Antibody
Question
I was wanting to use your anti-PARP-1 antibody for IP for mouse cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
It shows on the product datasheet, A00122-1 anti-PARP-1 antibody has been validated for ELISA, IP, IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-17
Question
Is a blocking peptide available for product anti-PARP-1 antibody (A00122-1)?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
We do provide the blocking peptide for product anti-PARP-1 antibody (A00122-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-11
Question
My colleagues were well pleased with the WB result of your anti-PARP-1 antibody. However we have been able to see positive staining in leukemic t-cell nucleus using this antibody. Is that expected? Could you tell me where is PARP1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
Based on literature, leukemic t-cell does express PARP1. Generally PARP1 expresses in nucleus. Regarding which tissues have PARP1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, and Ovarian carcinoma, Pubmed ID: 17177976
Embryonic kidney, Pubmed ID: 17525332
Fibroblast, Pubmed ID: 3120710, 2824474, 2891139
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2020-01-27
Question
Can you help my question with product A00122-1, anti-PARP-1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00122-1 anti-PARP-1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-31
Question
We are interested in to test anti-PARP-1 antibody A00122-1 on mouse cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-PARP-1 antibody A00122-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
The products we sell, including anti-PARP-1 antibody A00122-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-16
Question
Will A00122-1 anti-PARP-1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
It shows on the product datasheet, A00122-1 anti-PARP-1 antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-31
Question
Is this A00122-1 anti-PARP-1 antibody reactive to the isotypes of PARP1?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
The immunogen of A00122-1 anti-PARP-1 antibody is Recombinant human PARP-1 (poly (ADP-ribose) polymerase-1) (aa 1-1014) . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-15
Question
We are currently using anti-PARP-1 antibody A00122-1 for mouse tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
The anti-PARP-1 antibody (A00122-1) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-12
Question
Do you have a BSA free version of anti-PARP-1 antibody A00122-1 available?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PARP-1 antibody A00122-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-19
Question
We have seen staining in mouse leukemic t-cell. Any tips? Is anti-PARP-1 antibody supposed to stain leukemic t-cell positively?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
According to literature leukemic t-cell does express PARP1. According to Uniprot.org, PARP1 is expressed in heart left ventricle, fibroblast, brain, colon carcinoma ovarian carcinoma, embryonic kidney, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have PARP1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, and Ovarian carcinoma, Pubmed ID: 17177976
Embryonic kidney, Pubmed ID: 17525332
Fibroblast, Pubmed ID: 3120710, 2824474, 2891139
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-06-06
Question
We purchased anti-PARP-1 antibody for ELISA on cervix carcinoma a few months ago. I am using human, and We want to use the antibody for WB next. I am interested in examining cervix carcinoma as well as cervix carcinoma erythroleukemia in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
I viewed the website and datasheets of our anti-PARP-1 antibody and I see that A00122-1 has been validated on human in both ELISA and WB. Thus A00122-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-23
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-PARP-1 antibody A00122-1. Please let me know if you require anything else.
P. Thomas
Verified customer
Asked: 2018-09-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-27
Question
I see that the anti-PARP-1 antibody A00122-1 works with IP, what is the protocol used to produce the result images on the product page?
M. Mangal
Verified customer
Asked: 2018-09-20
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-09-20
Question
Would anti-PARP-1 antibody A00122-1 work for IP with cervix carcinoma erythroleukemia?
Z. Wu
Verified customer
Asked: 2018-06-26
Answer
According to the expression profile of cervix carcinoma erythroleukemia, PARP1 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-PARP-1 antibody A00122-1 will work for IP with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2018-06-26
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-PARP-1 antibody A00122-1. Let me know if you need anything else.
C. Bhatt
Verified customer
Asked: 2017-10-13
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-13
