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Product Info Summary
| SKU: | PB9837 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TRPM8 Antibody Picoband®
SKU/Catalog Number
PB9837
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRPM8 Antibody Picoband® catalog # PB9837. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRPM8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9837)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TRPM8, different from the related mouse sequence by four amino acids, and from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9837 is reactive to TRPM8 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
127 kDa
Calculated molecular weight
127685 MW
Background of TRPM8
Transient receptor potential cation channel subfamily M member 8 (TRPM8), also known as the cold and menthol receptor 1 (CMR1), is a protein that in humans is encoded by the TRPM8 gene. TRPM8 is an ion channel, upon activation it allows the entry of Na+ (sodium) and Ca2+ (calcium) ions to the cell that leads to depolarization and the generation of an action potential. The signal is conducted from primary afferents (type C- and A-delta) eventually leading to the sensation of cold and cold pain. The TRPM8 protein is expressed in sensory neurons, and it is activated by cold temperatures and cooling agents, such as menthol and icilin whereas WS-12 and CPS-369 are the most selective agonist of TRPM8.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9837 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA whole cell, 22RV1 whole cell, SW620 whole cell, A549 whole cell, A431 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TRPM8 using anti-TRPM8 antibody (PB9837).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: 22RV1 whole cell lysates,
Lane 3: SW620 whole cell lysates,
Lane 4: A549 whole cell lysates,
Lane 5: A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM8 antigen affinity purified polyclonal antibody (Catalog # PB9837) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM8 at approximately 127 kDa. The expected band size for TRPM8 is at 127 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TRPM8 (Source: Uniprot.org, NCBI)
Gene Name
TRPM8
Full Name
Transient receptor potential cation channel subfamily M member 8
Weight
127685 MW
Superfamily
transient receptor (TC 1.A.4) family
Alternative Names
Transient receptor potential cation channel subfamily M member 8;Long transient receptor potential channel 6;LTrpC-6;LTrpC6;Transient receptor potential p8;Trp-p8;TRPM8;LTRPC6, TRPP8; TRPM8 LTRPC6, LTrpC-6, TRPP8, trp-p8 transient receptor potential cation channel subfamily M member 8 transient receptor potential cation channel subfamily M member 8|TRPM8 cationic channel|transient receptor potential p8|transient receptor potential subfamily M member 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRPM8, check out the TRPM8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRPM8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRPM8 Antibody Picoband® (PB9837)
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Customer Q&As
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7 Customer Q&As for Anti-TRPM8 Antibody Picoband®
Question
Would PB9837 anti-TRPM8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
You can see on the product datasheet, PB9837 anti-TRPM8 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-20
Question
Is this PB9837 anti-TRPM8 antibody reactive to the isotypes of TRPM8?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
The immunogen of PB9837 anti-TRPM8 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human TRPM8 (1068-1104aa NTKANDTSEEMRHRFRQLDTKLNDLKGLLKEIANKIK), different from the related mouse sequence by four amino acids, and from the related rat sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-07
Question
Do you have a BSA free version of anti-TRPM8 antibody PB9837 available?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TRPM8 antibody PB9837 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-20
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for prostate using anti-TRPM8 antibody PB9837. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-07
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-07
Question
We are currently using anti-TRPM8 antibody PB9837 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-20
Answer
The anti-TRPM8 antibody (PB9837) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-20
Question
I was wanting to use your anti-TRPM8 antibody for WB for human prostate on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human prostate identification?
B. Miller
Verified customer
Asked: 2015-11-17
Answer
As indicated on the product datasheet, PB9837 anti-TRPM8 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human prostate in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-11-17
Question
Will anti-TRPM8 antibody PB9837 work for WB with prostate?
M. Wu
Verified customer
Asked: 2014-07-17
Answer
According to the expression profile of prostate, TRPM8 is highly expressed in prostate. So, it is likely that anti-TRPM8 antibody PB9837 will work for WB with prostate.
Boster Scientific Support
Answered: 2014-07-17
