Click image to see more details
Product Info Summary
| SKU: | A01002 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-TRPM8 Antibody (Center)
SKU/Catalog Number
A01002
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-TRPM8 Antibody (Center) (Catalog # A01002). Tested in WB, IF, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-TRPM8 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01002)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This TRPM8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263-292 amino acids from the Central region of human TRPM8.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01002 is reactive to TRPM8 in Human
Applications
A01002 is guaranteed for IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
127685 Da
Background of TRPM8
Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
IHC-P: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Confocal immunofluorescent analysis of TRPM8 Antibody (Center) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Click image to see more details
Western blot analysis of lysate from LNCaP cell line,using TRPM8 Antibody (Center) (Cat. #A01002). A01002 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
Click image to see more details
TRPM8 Antibody (Center) (Cat. #A01002)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TRPM8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Protein Target Info & Infographic
Gene/Protein Information For TRPM8 (Source: Uniprot.org, NCBI)
Gene Name
TRPM8
Full Name
Transient receptor potential cation channel subfamily M member 8
Weight
127685 Da
Superfamily
transient receptor (TC 1.A.4) family
Alternative Names
CMR1; Long transient receptor potential channel 6; LTRPC6; LTrpC-6; MGC2849; short form of the TRPM8 cationic channel; transient receptor potential cation channel, subfamily M, member 8; Transient receptor potential p8; transient receptor potential subfamily M member 8; TRPM8; TRPP8; trp-p8; TRPP8transient receptor potential cation channel subfamily M member 8 TRPM8 LTRPC6, LTrpC-6, TRPP8, trp-p8 transient receptor potential cation channel subfamily M member 8 transient receptor potential cation channel subfamily M member 8|TRPM8 cationic channel|transient receptor potential p8|transient receptor potential subfamily M member 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRPM8, check out the TRPM8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRPM8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRPM8 Antibody (Center) (A01002)
Hello CJ!
No publications found for A01002
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-TRPM8 Antibody (Center)?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-TRPM8 Antibody (Center)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
