Anti-TRPM2 Antibody Picoband™

Product Name

Anti-TRPM2 Antibody Picoband™

View all TRPM2 Antibodies

SKU/Catalog Number

A01013-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TRPM2 Antibody Picoband™ catalog # A01013-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-TRPM2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01013-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human TRPM2 recombinant protein (Position: R398-Y1503).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01013-1 is reactive to TRPM2 in Human, Mouse, Rat

Applications

A01013-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

171 kDa

Calculated molecular weight

63173 MW

Background of TRPM2

Transient receptor potential cation channel subfamily M member 2, also known as TRPM2 is a protein that in humans is encoded by the TRPM2 gene. Using a cosmid/BAC contig, this gene is mapped to chromosome 21q22.3. The protein encoded by this gene is a calcium-permeable cation channel that is regulated by free intracellular ADP-ribose. The encoded protein is activated by oxidative stress and confers susceptibility to cell death. Several alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of TRPM2 using anti-TRPM2 antibody (A01013-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM2 antigen affinity purified polyclonal antibody (Catalog # A01013-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM2 at approximately 171 kDa. The expected band size for TRPM2 is at 171 kDa.

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Figure 2. Flow Cytometry analysis of THP-1 cells using anti-TRPM2 antibody (A01013-1).
Overlay histogram showing THP-1 cells stained with A01013-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPM2 Antibody (A01013-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For TRPM2 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.6.1.13; EREG1MGC133383; Estrogen-responsive element-associated gene 1 protein; KNP3LTrpC-2; Long transient receptor potential channel 2; LTrpC2; LTRPC2TRPC7transient receptor potential cation channel subfamily M member 2; NUDT9H; NUDT9L1; transient receptor potential cation channel, subfamily M, member 2; Transient receptor potential channel 7; TrpC7 TRPM2 EREG1, KNP3, LTRPC2, LTrpC-2, NUDT9H, NUDT9L1, TRPC7 transient receptor potential cation channel subfamily M member 2 transient receptor potential cation channel subfamily M member 2|estrogen-responsive element-associated gene 1 protein|long transient receptor potential channel 2|transient receptor potential channel 7|transient receptor potential melastatin 2

For more info on TRPM2, check out the TRPM2 Infographic

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In this infographic, you will see the following information for TRPM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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