TRPM2 Antibodies

Transient receptor potential cation channel subfamily M member 2 (TRPM2)

Isoform 1: Nonselective, voltage-independent cation channel that mediates Na(+) and Ca(2+) influx, leading to increased cytoplasmic Ca(2+) levels (PubMed:11960981, PubMed:12594222, PubMed:11385575, PubMed:11509734, PubMed:11804595, PubMed:15561722, PubMed:16601673, PubMed:19171771, PubMed:20660597, PubMed:27383051, PubMed:27068538). Extracellular calcium passes through the station and increases channel activity by binding to the cytoplasmic domain and stabilizing the station in an open conformation (PubMed:19171771).

Plays a role in numerous procedures that involve signaling via intracellular Ca(2+) levels (Probable). Besides, mediates the release of lysosomal Zn(2+) stores in response to reactive oxygen species, leading to increased cytosolic Zn(2+) levels (PubMed:25562606, PubMed:27068538).

Gene Information

Human
Gene Name:	TRPM2
Uniprot:	O94759
Entrez:	7226

Family

Belongs to:
transient receptor (TC 1.A.4) family

Alternative Names

EC 3.6.1.13; EREG1MGC133383; Estrogen-responsive element-associated gene 1 protein; KNP3LTrpC-2; Long transient receptor potential channel 2; LTrpC2; LTRPC2TRPC7transient receptor potential cation channel subfamily M member 2; NUDT9H; NUDT9L1; transient receptor potential cation channel, subfamily M, member 2; Transient receptor potential channel 7; TrpC7

Molecular Weight

Mass (kDA):
171.198 kDA

Genomic Context

Human
Location:	21q22.3
Sequence:	21; NC_000021.9 (44350112..44443081)

Tissue Specificity

Highly expressed in brain and peripheral blood cells, such as neutrophils. Also detected in bone marrow, spleen, heart, liver and lung. Isoform 2 is found in neutrophil granulocytes.

Subcellular Localizations

Cell membrane; Multi-pass membrane protein. Perikaryon. Cell projection. Cytoplasmic vesicle. Lysosome. Detected at the cell membrane and in intracellular vesicles in cortical neurons. Detected on neuronal cell bodies and neurites (By similarity). Detected on the cell membrane in polymorphonuclear neutrophils. Detected on cytoplasmic vesicles and lysosomes in immature bone marrow dendritic cells (By similarity).; [Isoform 1]: Cell membrane; Multi-pass membrane protein.; [Isoform 2]: Cell membrane; Multi-pass membrane protein.; [Isoform 3]: Cell membrane; Multi-pass membrane protein.

Diseases

• Inflammation
• Neurodegenerative Disorders
• Bipolar Disorder
• Diabetes Mellitus
• Malignant Neoplasms
• Insulinoma
• Brain Injuries
• Pain
• Nervousness
• Neoplasms
• Mood Disorders
• Carcinoma
• Edema

• Cerebrovascular Accident
• Leukemia
• Alzheimer's Disease
• Lung Diseases
• Ischemia
• Diabetes Mellitus, Non-insulin-dependent
• Neuralgia

Pathways

• Cell Death
• Secretion
• Insulin Secretion
• Cell Proliferation
• Localization
• Pathogenesis
• Response To Oxidative Stress
• Cell Migration
• Chemotaxis
• Membrane Depolarization
• Cell Activation
• Aging
• Neuroprotection

• Rna Interference
• Chemokine Production
• T Cell Activation
• Immune Response
• Cytokine Secretion
• Inflammatory Response
• Store-operated Calcium Entry

PTMs

• Phosphorylation
• Cleavage
• Glycosylation
• Biotinylation
• Reduction

• Acetylation
• Methylation
• Oxidation
• Ribosylation
• Palmitoylation

Research Areas

• Apoptosis
• Cell Biology
• Membrane Trafficking and Chaperones
• Neuronal Cell Markers
• Neuroscience

• Neurotransmission
• Signal Transduction

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TRPM2

Best Uses of the TRPM2 Marker

The TRPM2 Marker is an innovative tool that was designed to allow researchers to measure the level of proteins in biological samples. Boster scientists can utilize it to test different species, applications as well as special samples and also receive credit for the product. Its versatility permits scientists to test proteins in a variety of biological systems, including bacteria and fungi. Researchers from all over the world could benefit from its advantages.

Benefits of using the TRPM2 Marker

The TRPM2 Marker is a potential candidate for the development of a new therapeutic target for the treatment of kidney diseases. It is an essential component of the cellular processes which regulates the longevity. TRPM2 was studied in mice with AKI and renal fibrosis. The genes were administered to animals and the animals' responses to heat stress were analyzed. In addition, TRPM2-knockout mice were used in studies to determine if the protein is necessary for renal fibrosis or AKI.

Studies have revealed that TRPM2 plays a significant role in keeping the NPC pool healthy under heat stress. These results provide a framework to study neurological disorders. Further research is required to establish the function of TRPM2 in the brain. The TRPM2 Marker could be a more effective diagnostic tool. Benefits of the TRPM2 marker

Common uses

The TRPM2 marker is expressed on a broad variety of immune cells and regulates the bactericidal function of phagocytic cells. The expression of TRPM2 was found to be increased in various types of cancer. However, it was found to be most prevalent in renal clear cell carcinoma. Despite its many uses it is known that the TRPM2 marker has a few limitations, including its sensitivity and specificity. We will discuss some of the most common uses for this marker.

The TRPM2 gene encodes a protein which is a Ca2+-permeableand non-covalent channel. The protein is found in a variety cell types that include microglia, neuronal, lung cells, immune cells and B cells. However, its precise role remains a mystery. While it has been utilized in numerous studies to investigate the mechanisms behind neuropathy-related pain, further research is needed.

It is used to detect the presence of oxidative stress and trigger Ca2+ influx through TRPM2. The resulting signal triggers the CAMK2 enzyme, and blocks autophagy. This makes cells more vulnerable to cell death. Additionally, it is used to detect the toxicity of hypoxia. It is also used to monitor inflammation and cancer. However, it must be taken into consideration that TRPM2 expression in cancer cells should be taken with caution.

Specific applications

TRPM2 is a crucial channel that plays a critical role in the death of cells particularly in the context of TNF-a and oxidative stress. This marker is used in a variety of applications, including cancer, cardiovascular disease, and neurodegenerative diseases. It could be one day targeted as a therapeutic strategy. However, it is far from being the only candidate. There are many other uses of this marker. Here are a few of them:

The receptor is expressed on the surface of NK cells that have received an IL-2-stimulated or TNF-a-stimulated ambiance. It can be used to determine if NK cells are active and for other immune-related research. Although the TRP-ion channels are not widely used and is often neglected, it is crucial in identifying the subsets of cancerous cells.

Studies with siRNA targeting the TRPM2 gene showed that the siRNA may reduce the rate of apoptosis in a variety of cancer cells. One study involved transfection of cells with either an empty vector or siRNA targeting TRPM2 and expressing luciferase. The resulting changes were identical in triplicates. There are numerous advantages to using RNAi for cancer treatment. One of these benefits is the capability to use RNAi in the culture of cells to identify cancer cells that already express the protein.

The TRPM2 marker can be used as an indicator for KIRC. The protein also regulates the activation of T-cells. As research continues, more details about the role of the receptor in the field of cancer research will be revealed. And there is also potential for this marker in the process of immune cell infiltration. Its role isn't clear. Specific applications of the TRPM2 marker are in the early stages of development.

TRPM2 has been shown to be a biomarker that detects the stress of oxidative. Overly high oxidative stress can lead to the formation of aggregated proteins which can cause a variety of diseases. Oxidative stress can also promote apoptosis. In addition to aging the accumulation of these aggregated substances is associated with neurodegeneration. TRPM2 is also an enzyme that assists in the elimination of ADPR.

The pSUPER vector was generated by using a retroviral vector (pCMV-Script) which contains an RNAi targeted at the TRPM2 gene. It also works to reduce the expression of the luciferase enzyme. This gene has been found to be present in the retroviral vector demonstrating that the RNAi was effective in the infection of the cells. A neomycin-resistant gene also was discovered in the retroviral virus vector.