Anti-TRP 7/TRPC7 Antibody Picoband™

Product Name

Anti-TRP 7/TRPC7 Antibody Picoband™

View all TRP7 Antibodies

SKU/Catalog Number

A04658-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TRP 7/TRPC7 Antibody Picoband™ catalog # A04658-2. Tested in ELISA, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-TRP 7/TRPC7 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04658-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human TRP 7/TRPC7 recombinant protein (Position: R489-I862).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04658-2 is reactive to Trpc7 in Human, Rat

Applications

A04658-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

100 kDa

Calculated molecular weight

Background of TRP7

Transient receptor potential cation channel, subfamily C, member 7, also known as TRPC7, is a human gene encoding a protein of the same name. Using a cosmid/BAC contig, this gene is mapped to chromosome 21q22.3. TRPC7 is the most abundantly expressed long TRPC in peripheral blood and in some blood cell lines. The protein encoded by this gene is a calcium-permeable cation channel that is regulated by free intracellular ADP-ribose. The encoded protein is activated by oxidative stress and confers susceptibility to cell death.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of TRP 7/TRPC7 using anti-TRP 7/TRPC7 antibody (A04658-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human SK-N-SH whole cell lysates,
Lane 5: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRP 7/TRPC7 antigen affinity purified polyclonal antibody (Catalog # A04658-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRP 7/TRPC7 at approximately 100 kDa. The expected band size for TRP 7/TRPC7 is at 100 kDa.

Gene/Protein Information For Trpc7 (Source: Uniprot.org, NCBI)

Alternative Names

hTRP7; KNP3; likley ortholog of mouse transient receptor potential cation channel, subfamilyC, member 7; putative capacitative calcium channel; short transient receptor potential channel 7; transient receptor potential cation channel, subfamily C, member 7; transient receptor potential-related channel 7, a novel putative Ca2+ channelprotein10TRPM2; Transient receptor protein 7; TRP7; TRP-7; TrpC7 Trpc7|TR, TRP7, Trrp8|transient receptor potential cation channel, subfamily C, member 7|short transient receptor potential channel 7|transient receptor potential protein homologue TRP7|transient receptor protein 7|transient receptor protein 8

For more info on Trpc7, check out the Trpc7 Infographic

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In this infographic, you will see the following information for Trpc7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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