Product Info Summary
SKU: | A06030-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-TRNT1 Antibody Picoband™
SKU/Catalog Number
A06030-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRNT1 Antibody Picoband™ catalog # A06030-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TRNT1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06030-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TRNT1 recombinant protein (Position: M1-Q410).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06030-1 is reactive to TRNT1 in Human
Applications
A06030-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
50 kDa
Calculated molecular weight
80420 MW
Background of TRNT1
tRNA-nucleotidyltransferase 1, is an enzyme that in humans is encoded by the TRNT1 gene. The protein encoded by this gene is a CCA-adding enzyme which belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. This essential enzyme functions by catalyzing the addition of the conserved nucleotide triplet CCA to the 3' terminus of tRNA molecules. Mutations in this gene result in sideroblastic anemia with B-cell immunodeficiency, periodic fevers, and developmental delay. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
![a06030 1 trnt1 primary antibodies wb testing 1 a06030 1 trnt1 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a06030-1-trnt1-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of TRNT1 using anti-TRNT1 antibody (A06030-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRNT1 antigen affinity purified polyclonal antibody (Catalog # A06030-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRNT1 at approximately 50 kDa. The expected band size for TRNT1 is at 50 kDa.
![a06030 1 trnt1 primary antibodies ihc testing 2 a06030 1 trnt1 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a06030-1-trnt1-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of TRNT1 using anti-TRNT1 antibody (A06030-1).
TRNT1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRNT1 Antibody (A06030-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a06030 1 trnt1 primary antibodies ihc testing 3 a06030 1 trnt1 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a06030-1-trnt1-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of TRNT1 using anti-TRNT1 antibody (A06030-1).
TRNT1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRNT1 Antibody (A06030-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a06030 1 trnt1 primary antibodies ihc testing 4 a06030 1 trnt1 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a06030-1-trnt1-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of TRNT1 using anti-TRNT1 antibody (A06030-1).
TRNT1 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRNT1 Antibody (A06030-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a06030 1 trnt1 primary antibodies ihc testing 5 a06030 1 trnt1 primary antibodies ihc testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a06030-1-trnt1-primary-antibodies-ihc-testing-5.jpg)
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Figure 5. IHC analysis of TRNT1 using anti-TRNT1 antibody (A06030-1).
TRNT1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRNT1 Antibody (A06030-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For TRNT1 (Source: Uniprot.org, NCBI)
Gene Name
TRNT1
Full Name
CCA tRNA nucleotidyltransferase 1, mitochondrial
Weight
80420 MW
Superfamily
tRNA nucleotidyltransferase/poly(A) polymerase family
Alternative Names
CCA1; CCA-adding; CGI-47; EC 2.7.7.72; mitochondrial CCA-adding tRNA-nucleotidyltransferase; mt CCA-adding enzyme; mt tRNA adenylyltransferase; mt tRNA CCA-diphosphorylase; mt tRNA CCA-pyrophosphorylase; MtCCA; tRNA nucleotidyl transferase, CCA-adding, 1; tRNA-nucleotidyltransferase 1, mitochondrial TRNT1 CCA1, CGI-47, MtCCA, RPEM, SIFD tRNA nucleotidyl transferase 1 CCA tRNA nucleotidyltransferase 1, mitochondrial|ATP(CTP):tRNA nucleotidyltransferase|CCA-adding enzyme|mitochondrial CCA-adding tRNA-nucleotidyltransferase|mt CCA-adding enzyme|mt tRNA CCA-diphosphorylase|mt tRNA CCA-pyrophosphorylase|mt tRNA adenylyltransferase|tRNA CCA nucleotidyl transferase 1|tRNA nucleotidyl transferase, CCA-adding, 1|tRNA-nucleotidyltransferase 1, mitochondrial
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRNT1, check out the TRNT1 Infographic
![TRNT1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRNT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRNT1 Antibody Picoband™ (A06030-1)
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