Product Info Summary
SKU: | A11359-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-TRMT5 Antibody Picoband™
SKU/Catalog Number
A11359-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRMT5 Antibody Picoband™ catalog # A11359-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TRMT5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11359-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TRMT5 recombinant protein (Position: R291-E501).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A11359-1 is reactive to TRMT5 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
Background of TRMT5
tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11359-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Positive Control
Validation Images & Assay Conditions
![a11359 1 trmt5 primary antibodies wb testing 1 a11359 1 trmt5 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/1/a11359-1-trmt5-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of TRMT5 using anti-TRMT5 antibody (A11359-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRMT5 antigen affinity purified polyclonal antibody (Catalog # A11359-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRMT5 at approximately 50 kDa. The expected band size for TRMT5 is at 58 kDa.
![a11359 1 trmt5 primary antibodies if testing 2 a11359 1 trmt5 primary antibodies if testing 2](https://www.bosterbio.com/media/catalog/product/a/1/a11359-1-trmt5-primary-antibodies-if-testing-2.jpg)
Click image to see more details
Figure 2. IF analysis of TRMT5 using anti-TRMT5 antibody (A11359-1) and anti-Tubulin Alpha antibody (M03989-3).
TRMT5 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TRMT5 Antibody (A11359-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For TRMT5 (Source: Uniprot.org, NCBI)
Gene Name
TRMT5
Full Name
tRNA (guanine(37)-N1)-methyltransferase
Weight
Superfamily
class I-like SAM-binding methyltransferase superfamily
Alternative Names
KIAA1393EC 2.1.1.31; M1G-methyltransferase; TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae); TRM5MGC111453; tRNA (guanine-N(1)-)-methyltransferase; tRNA [GM37] methyltransferase; tRNA methyltransferase 5; tRNA-N1G37 methyltransferase TRMT5 COXPD26, KIAA1393, TRM5 tRNA methyltransferase 5 tRNA (guanine(37)-N1)-methyltransferase|M1G-methyltransferase|TRM5 tRNA methyltransferase 5 homolog|tRNA (guanine-N(1)-)-methyltransferase|tRNA [GM37] methyltransferase|tRNA-N1G37 methyltransferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRMT5, check out the TRMT5 Infographic
![TRMT5 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRMT5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRMT5 Antibody Picoband™ (A11359-1)
Hello CJ!
No publications found for A11359-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-TRMT5 Antibody Picoband™?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-TRMT5 Antibody Picoband™
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question