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Product Info Summary
| SKU: | A08005 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-TRIM71 Antibody
View all LIN-41/TRIM71 Antibodies
SKU/Catalog Number
A08005
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TRIM71 Antibody (Catalog # A08005). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TRIM71 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-TRIM71 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08005)
Host
Rabbit
Contents
TRIM71 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TRIM71 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human TRIM71. The immunogen is located within amino acids 680 - 730 of TRIM71.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
RBM35B antibody is predicted to not cross-react with other RBM35/ESRP family members. At least two isoforms of RBM35B are known to exist; this antibody will detect both isoforms.
Reactive Species
A08005 is reactive to TRIM71 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
93385 MW
Background of LIN-41/TRIM71
TRIM71 is the mammalian ortholog to the C. elegans heterochronic protein Lin41, a protein that is thought to be important in postembryonic development, and is genetically and biochemically downstream of both Shh and Fgf signaling pathways. Similar to its C. elegans homolog, TRIM71 is a target of the microRNA let-7 and is likely to play a role in mammalian development. Recent experiments have indicated that TRIM71 is an E3 ubiquitin ligase and can interact with the Dicer and the Argonaute proteins Ago1, Ago2, and Ago4. Overexpression and depletion of TRIM71 led to inverse changes in Ago2 protein levels, suggesting TRIM71 can regulate Ago2 turnover. Finally, TRIM71 cooperates with the pluripotency factor Lin-28 in regulating let-7.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08005 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TRIM71 antibody can be used for detection of TRIM71 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TRIM71 in human brain tissue lysate with TRIM71 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of TRIM71 in human brain tissue with TRIM71 antibody at 5 μg/mL.
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Immunofluorescence of TRIM71 in Human Brain cells with TRIM71 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For TRIM71 (Source: Uniprot.org, NCBI)
Gene Name
TRIM71
Full Name
E3 ubiquitin-protein ligase TRIM71
Weight
93385 MW
Superfamily
TRIM/RBCC family
Alternative Names
LIN41, LIN-41, LIN41, E3 ubiquitin-protein ligase TRIM71, Protein lin-41 homolog TRIM71 HYDCC1, LIN-41, LIN41 tripartite motif containing 71 E3 ubiquitin-protein ligase TRIM71|RING-type E3 ubiquitin transferase TRIM71|abnormal cell LINeage LIN-41|homolog of C. elegans Lin-41|protein lin-41 homolog|tripartite motif containing 71, E3 ubiquitin protein ligase|tripartite motif-containing protein 71
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRIM71, check out the TRIM71 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRIM71: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRIM71 Antibody (A08005)
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