Anti-TRIM33 Antibody

Product Name

Anti-TRIM33 Antibody

View all TIF1 gamma Antibodies

SKU/Catalog Number

A03133

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TRIM33 Antibody (Catalog # A03133). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

TRIM33 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-TRIM33 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03133)

Host

Rabbit

Contents

TRIM33 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TRIM33 antibody was raised against an 18 amino acid peptide near the center of human TRIM33. The immunogen is located within amino acids 580 - 630 of TRIM33.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

TRIM33 antibody is human, mouse and rat reactive. At least two isoforms of TRIM33 are known to exist.

Reactive Species

A03133 is reactive to TRIM33 in Human, Mouse, Rat

Applications

A03133 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

122533 MW

Background of TIF1 gamma

TRIM24 (TIF1alpha), TRIM28 (TIF1beta), and TRIM33 (TIF1gamma) are three related cofactors belonging to the tripartite motif superfamily that interact with distinct transcription factors (1-2). The structure of TRIM33 is similar to TRIM24 and TRIM28, exhibiting multiple domains (RING finger, coiled coil, B boxes, PHD/TTC, and bromodomain) (3). TRIM33 functions in cell differentiation and development, playing a role in differentiation of hematopoietic cells (3). It interacts with the Smad2/3 transcription factor in hematopoietic, mesenchymal, and epithelial cell types to mediate different transcriptional effects in response to TGF-beta (4). It acts as an E3 ubiquitin-protein ligase and promotes SMAD4 ubiquitination (5).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TRIM33 antibody can be used for detection of TRIM33 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of TRIM33 in human liver tissue lysate with TRIM33 antibody at (A) 1 and (B) 2 μg/mL.

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Immunohistochemistry of TRIM33 in human liver tissue with TRIM33 antibody at 2.5 μg/ml.

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Immunofluorescence of TRIM33 in human liver tissue with TRIM33 antibody at 20 μg/ml.

Gene/Protein Information For TRIM33 (Source: Uniprot.org, NCBI)

Alternative Names

E3 ubiquitin-protein ligase TRIM33; EC 6.3.2; EC 6.3.2.-; Ectodermin homolog; KIAA1113; Protein Rfg7; PTC7FLJ32925; RET-fused gene 7 protein; RFG7FLJ11429; TF1G; TIF1GAMMA; TIF1-gamma; TIF1GECTO; TIFGAMMA; Transcription intermediary factor 1-gamma; transcriptional intermediary factor 1 gamma; tripartite motif containing 33; tripartite motif-containing 33; Tripartite motif-containing protein 33 TRIM33 ECTO, PTC7, RFG7, TF1G, TIF1G, TIF1GAMMA, TIFGAMMA tripartite motif containing 33 E3 ubiquitin-protein ligase TRIM33|RET-fused gene 7 protein|RING-type E3 ubiquitin transferase TRIM33|TIF1-gamma|ectodermin homolog|protein Rfg7|transcriptional intermediary factor 1 gamma

For more info on TRIM33, check out the TRIM33 Infographic

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In this infographic, you will see the following information for TRIM33: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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