Anti-TRIM24/Tif1 Alpha Antibody

Product Name

Anti-TRIM24/Tif1 Alpha Antibody

View all TIF1 alpha Antibodies

SKU/Catalog Number

A03258-1

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-TRIM24/Tif1 Alpha Antibody (Catalog # A03258-1). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TRIM24/Tif1 Alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03258-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from C-terminal of human TRIM24.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03258-1 is reactive to TRIM24 in Human, Mouse

Reconstitution

Calculated molecular weight

116831 MW

Background of TIF1 alpha

Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes By similarity.

Thenot S., J. Biol. Chem. 272:12062-12068(1997).
Venturini L., Oncogene 18:1209-1217(1999).
Hellal-Levy C., Mol. Endocrinol. 14:1210-1221(2000).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03258-1 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

Gene/Protein Information For TRIM24 (Source: Uniprot.org, NCBI)

Alternative Names

Transcription intermediary factor 1-alpha;TIF1-alpha;6.3.2.-;E3 ubiquitin-protein ligase TRIM24;RING finger protein 82;Tripartite motif-containing protein 24;TRIM24;RNF82, TIF1, TIF1A; TRIM24 PTC6, RNF82, TF1A, TIF1, TIF1A, TIF1ALPHA, hTIF1 tripartite motif containing 24 transcription intermediary factor 1-alpha|E3 ubiquitin-protein ligase TRIM24|RING finger protein 82|RING-type E3 ubiquitin transferase TIF1-alpha|TIF1-alpha|transcriptional intermediary factor 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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