Product Info Summary
SKU: | A02079-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-TRIM21/SS-A Antibody Picoband™
View all TRIM21/SSA1 Antibodies
SKU/Catalog Number
A02079-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRIM21/SS-A Antibody Picoband™ catalog # A02079-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRIM21/SS-A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02079-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TRIM21/SS-A recombinant protein (Position: E12-Y475).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02079-2 is reactive to TRIM21 in Human, Mouse, Rat
Applications
A02079-2 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
52 kDa
Calculated molecular weight
Background of TRIM21/SSA1
Tripartite motif-containing protein 21 also known as E3 ubiquitin-protein ligase TRIM21 is a protein that in humans is encoded by the TRIM21 gene. It is mapped to 11p15.4. This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TRIM21/SS-A using anti-TRIM21/SS-A antibody (A02079-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: mouse lung tissue lysates,
Lane 3: rat thymus tissue lysates,
Lane 4: mouse thymus tissue lysates,
Lane 5: human Hela whole cell lysates,
Lane 6: human THP-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM21/SS-A antigen affinity purified polyclonal antibody (Catalog # A02079-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM21/SS-A at approximately 52KD. The expected band size for TRIM21/SS-A is at 52KD.
Click image to see more details
Figure 2. IF analysis of TRIM21/SS-A using anti-TRIM21/SS-A antibody (A02079-2).
TRIM21/SS-A was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-TRIM21/SS-A Antibody (A02079-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 3. Flow Cytometry analysis of SiHa cells using anti-TRIM21/SS-A antibody (A02079-2).
Overlay histogram showing SiHa cells stained with A02079-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIM21/SS-A Antibody (A02079-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For TRIM21 (Source: Uniprot.org, NCBI)
Gene Name
TRIM21
Full Name
E3 ubiquitin-protein ligase TRIM21
Weight
Superfamily
TRIM/RBCC family
Alternative Names
52 kDa ribonucleoprotein autoantigen Ro/SS-A; 52 kDa Ro/SSA; E3 ubiquitin-protein ligase TRIM21; EC 6.3.2.-; rhTRIM21; RNF81; RNF81RING finger protein 81; ro(SS-A); Ro52; RO52SS-A; Sicca syndrome antigen A; Sjoegren syndrome type A antigen; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro); SSA1; SSA1SSA; TRIM21; tripartite motif containing 21,52 kDa Ro protein; tripartite motif-containing 21; Tripartite motif-containing protein 21 TRIM21 RNF81, RO52, Ro/SSA, SSA, SSA1 tripartite motif containing 21 E3 ubiquitin-protein ligase TRIM21|52 kDa Ro protein|52 kDa ribonucleoprotein autoantigen Ro/SS-A|RING finger protein 81|RING-type E3 ubiquitin transferase TRIM21|Ro/SSA 52kDa|SS-A|Sicca syndrome antigen A|Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro)|ro(SS-A)|sjoegren syndrome type A antigen|tripartite motif-containing protein 21
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRIM21, check out the TRIM21 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRIM21: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRIM21/SS-A Antibody Picoband™ (A02079-2)
Hello CJ!
A02079-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Min He,Si-Qi Zhao,Xi-Yan Guo et al.Effects of Terminalia chebula Retz. on the Secretion of Somatostatin in the Small Intestine in Mice,19 April 2021,PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-440387/v1]
Species: Mouse
A02079-2 usage in article: APP:IHC, SAMPLE:INTESTINAL TISSUE, DILUTION:1:100
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