Anti-CD80 Monoclonal Antibody [8G12]

Product Name

Anti-CD80 Monoclonal Antibody [8G12]

View all B7-1/CD80 Antibodies

SKU/Catalog Number

M00196-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-CD80 Monoclonal Antibody [8G12] (Catalog # M00196-1). Tested in ELISA, IHC-P, ICC, IF, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

CD80 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-CD80 Monoclonal Antibody [8G12] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00196-1)

Host

Mouse

Contents

CD80 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

Clonality

Monoclonal

Clone Number

Clone: 8G12

Isotype

IgG2b

Immunogen

CD80 antibody was raised against the extracellular domain of human CD80.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

PD-L1 Antibody has no cross-reactivity to PD-L2.

Reactive Species

M00196-1 is reactive to CD80 in Human

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

33048 MW

Background of B7-1/CD80

CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00196-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC-P, ICC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

CD80 antibody can be used for immunohistochemistry starting at 2 - 5 μg/mL. For immunofluorescence start at 5 μg/mL. Flow cytometry at 10 μg/ml.
Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For CD80 (Source: Uniprot.org, NCBI)

Alternative Names

CD80 molecule, B7, BB1, B7-1, B7.1, LAB7, CD28LG, CD28LG1 CD80 B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7 CD80 molecule T-lymphocyte activation CD80|B-lymphocyte activation B7|CD80 (CD28 ligand 1, B7-1 )|CTLA-4 counter-receptor B7.1|activation B7-1 |costimulatory factor CD80|costimulatory molecule variant IgV-CD80

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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