Click image to see more details
Product Info Summary
| SKU: | A09514 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-TRIM16 Antibody
SKU/Catalog Number
A09514
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-TRIM16 Antibody (Catalog # A09514). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRIM16 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09514)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human TRIM16.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09514 is reactive to TRIM16 in Human
Reconstitution
Calculated molecular weight
63955 MW
Background of TRIM16
May play a role in the regulation of keratinocyte differentiation.
Liu H.-L.C., Mol. Endocrinol. 12:1733-1748(1998).
Beer H.-D., J. Biol. Chem. 277:20740-20749(2002).
Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09514 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human testis tissue, using TRIM16 antibody A09514.
Protein Target Info & Infographic
Gene/Protein Information For TRIM16 (Source: Uniprot.org, NCBI)
Gene Name
TRIM16
Full Name
Tripartite motif-containing protein 16
Weight
63955 MW
Superfamily
TRIM/RBCC family
Alternative Names
Tripartite motif-containing protein 16;Estrogen-responsive B box protein;TRIM16;EBBP; TRIM16 EBBP tripartite motif containing 16 tripartite motif-containing protein 16|E3 ubiquitin-protein ligase TRIM16|estrogen-responsive B box protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRIM16, check out the TRIM16 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRIM16: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRIM16 Antibody (A09514)
Hello CJ!
No publications found for A09514
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-TRIM16 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-TRIM16 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-TRIM16 Antibody
Question
We are currently using anti-TRIM16 antibody A09514 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
The anti-TRIM16 antibody (A09514) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-07
Question
Does A09514 anti-TRIM16 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
You can see on the product datasheet, A09514 anti-TRIM16 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-01
Question
I see that the anti-TRIM16 antibody A09514 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-06
Question
My question regarding product A09514, anti-TRIM16 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-12-13
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09514 anti-TRIM16 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-13
Question
Is there a BSA free version of anti-TRIM16 antibody A09514 available?
Verified Customer
Verified customer
Asked: 2018-03-27
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-TRIM16 antibody A09514 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-03-27
Question
Is this A09514 anti-TRIM16 antibody reactive to the isotypes of TRIM16?
M. Miller
Verified customer
Asked: 2016-05-10
Answer
The immunogen of A09514 anti-TRIM16 antibody is Synthesized peptide derived from N-terminal of human TRIM16. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-05-10
Question
See below the WB image, lot number and protocol we used for mammary gland using anti-TRIM16 antibody A09514. Please let me know if you require anything else.
B. Baker
Verified customer
Asked: 2013-08-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-08-15
