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Product Info Summary
| SKU: | A09398-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TRIL Antibody Picoband®
SKU/Catalog Number
A09398-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRIL Antibody Picoband® catalog # A09398-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TRIL Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09398-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TRIL recombinant protein (Position: V26-Q696).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09398-1 is reactive to TRIL in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
89 kDa
Calculated molecular weight
88.723kDa
Background of TRIL
KIAA0644, also known as TRIL or TLR4 interactor with leucine rich repeats, is a protein that in humans is encoded by the KIAA0644 gene. TRIL is a component of the TLR4 (603030) complex and is induced in a number of cell types by lipopolysaccharide (LPS).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09398-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TRIL using anti-TRIL antibody (A09398-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIL antigen affinity purified polyclonal antibody (Catalog # A09398-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIL at approximately 89 kDa. The expected band size for TRIL is at 89 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TRIL (Source: Uniprot.org, NCBI)
Gene Name
TRIL
Full Name
TLR4 interactor with leucine rich repeats
Weight
88.723kDa
Alternative Names
B-cell differentiation antigen CD72; Lyb-2; CD72; CD72 TRIL TLR4 interactor with leucine rich repeats TLR4 interactor with leucine rich repeats
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRIL, check out the TRIL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRIL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRIL Antibody Picoband® (A09398-1)
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