Click image to see more details
Product Info Summary
| SKU: | A00650-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-TRF2/TERF2 Antibody Picoband®
SKU/Catalog Number
A00650-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRF2/TERF2 Antibody Picoband® catalog # A00650-1. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRF2/TERF2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00650-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TRF2 recombinant protein (Position: A81-K287). Human TRF2 shares 95.7% amino acid (aa) sequence identity with mouse TRF2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A00650-1 is reactive to TERF2 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
75 kDa
Calculated molecular weight
59594 MW
Background of TRF-2
Telomeric repeat-binding factor 2, also known as TERF2, TRF2, or TRBF2, is encoded in humans by the TERF2 gene. This gene encodes a telomere specific protein, TERF2, which is a component of the telomere nucleoprotein complex. This protein is present at telomeres in metaphase of the cell cycle, is a second negative regulator of telomere length and plays a key role in the protective activity of telomeres. While having similar telomere binding activity and domain organization, TERF2 differs from TERF1 in that its N terminus is basic rather than acidic.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00650-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: rat thymus tissue, human COLO320 whole cell
IHC: human tonsil tissue, human lung cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TRF2 using anti-TRF2 antibody (A00650-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: human COLO320 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRF2 antigen affinity purified polyclonal antibody (Catalog # A00650-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRF2 at approximately 75 kDa. The expected band size for TRF2 is at 59KD.
Click image to see more details
Figure 2. IHC analysis of TRF2 using anti-TRF2 antibody (A00650-1).
TRF2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TRF2 Antibody (A00650-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of TRF2 using anti-TRF2 antibody (A00650-1).
TRF2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TRF2 Antibody (A00650-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For TERF2 (Source: Uniprot.org, NCBI)
Gene Name
TERF2
Full Name
Telomeric repeat-binding factor 2
Weight
59594 MW
Alternative Names
Telomeric repeat-binding factor 2;TTAGGG repeat-binding factor 2;Telomeric DNA-binding protein;TERF2;TRBF2, TRF2; TERF2 TRBF2, TRF2 telomeric repeat binding factor 2 telomeric repeat-binding factor 2|TTAGGG repeat-binding factor 2|telomeric DNA-binding protein|telomeric repeat binding protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TERF2, check out the TERF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TERF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRF2/TERF2 Antibody Picoband® (A00650-1)
Hello CJ!
No publications found for A00650-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-TRF2/TERF2 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-TRF2/TERF2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-TRF2/TERF2 Antibody Picoband®
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for frontal cortex using anti-TRF2/TERF2 antibody A00650-1. Let me know if you need anything else.
D. Jha
Verified customer
Asked: 2020-04-29
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-29
Question
We have seen staining in human liver. Do you have any suggestions? Is anti-TRF2/TERF2 antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
From literature liver does express TERF2. From Uniprot.org, TERF2 is expressed in frontal cortex, adrenal cortex, mammary carcinoma, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have TERF2 expression, here are a few articles citing expression in various tissues:
Adrenal cortex, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 8614633, 9326951, 16964243, 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 9326950
Boster Scientific Support
Answered: 2020-03-18
Question
I see that the anti-TRF2/TERF2 antibody A00650-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-12
Question
Is a blocking peptide available for product anti-TRF2/TERF2 antibody (A00650-1)?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
We do provide the blocking peptide for product anti-TRF2/TERF2 antibody (A00650-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-22
Question
Is this A00650-1 anti-TRF2/TERF2 antibody reactive to the isotypes of TERF2?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
The immunogen of A00650-1 anti-TRF2/TERF2 antibody is E.coli-derived human TRF2 recombinant protein (Position: A81-K287). Human TRF2 shares 95.7% amino acid (aa) sequence identity with mouse TRF2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-05
Question
I have a question about product A00650-1, anti-TRF2/TERF2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00650-1 anti-TRF2/TERF2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-15
Question
See below the WB image, lot number and protocol we used for frontal cortex using anti-TRF2/TERF2 antibody A00650-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-19
Question
I was wanting to use your anti-TRF2/TERF2 antibody for WB for rat frontal cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat frontal cortex identification?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
You can see on the product datasheet, A00650-1 anti-TRF2/TERF2 antibody has been validated for IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat frontal cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-13
Question
Does A00650-1 anti-TRF2/TERF2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
It shows on the product datasheet, A00650-1 anti-TRF2/TERF2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-17
Question
We are currently using anti-TRF2/TERF2 antibody A00650-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-20
Answer
The anti-TRF2/TERF2 antibody (A00650-1) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-20
Question
Will anti-TRF2/TERF2 antibody A00650-1 work for WB with frontal cortex?
Verified Customer
Verified customer
Asked: 2018-06-20
Answer
According to the expression profile of frontal cortex, TERF2 is highly expressed in frontal cortex. So, it is likely that anti-TRF2/TERF2 antibody A00650-1 will work for WB with frontal cortex.
Boster Scientific Support
Answered: 2018-06-20
Question
I would like to test anti-TRF2/TERF2 antibody A00650-1 on rat frontal cortex for research purposes, then I may be interested in using anti-TRF2/TERF2 antibody A00650-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Moore
Verified customer
Asked: 2017-06-14
Answer
The products we sell, including anti-TRF2/TERF2 antibody A00650-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-14
Question
Do you have a BSA free version of anti-TRF2/TERF2 antibody A00650-1 available?
G. Banerjee
Verified customer
Asked: 2017-02-13
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-TRF2/TERF2 antibody A00650-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-02-13
Question
Our lab want to know about using your anti-TRF2/TERF2 antibody for negative regulation of telomeric d-loop disassembly studies. Has this antibody been tested with western blotting on colo320 whole cell lysates? We would like to see some validation images before ordering.
K. Taylor
Verified customer
Asked: 2016-04-13
Answer
Thank you for your inquiry. This A00650-1 anti-TRF2/TERF2 antibody is tested on colo320 whole cell lysates. It is guaranteed to work for IHC, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-04-13
Question
We ordered your anti-TRF2/TERF2 antibody for IHC on cervix carcinoma erythroleukemia in a previous project. I am using rat, and We intend to use the antibody for WB next. We are interested in examining cervix carcinoma erythroleukemia as well as adrenal cortex in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
B. Li
Verified customer
Asked: 2014-04-02
Answer
I looked at the website and datasheets of our anti-TRF2/TERF2 antibody and it seems that A00650-1 has been validated on rat in both IHC and WB. Thus A00650-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-04-02
Question
Our team were well pleased with the WB result of your anti-TRF2/TERF2 antibody. However we have observed positive staining in frontal cortex nucleus using this antibody. Is that expected? Could you tell me where is TERF2 supposed to be expressed?
R. Wu
Verified customer
Asked: 2013-01-23
Answer
From literature, frontal cortex does express TERF2. Generally TERF2 expresses in nucleus. Regarding which tissues have TERF2 expression, here are a few articles citing expression in various tissues:
Adrenal cortex, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 8614633, 9326951, 16964243, 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 9326950
Boster Scientific Support
Answered: 2013-01-23
