Product Info Summary
SKU: | A03277 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-TRAP ACP5 Antibody
View all TRACP/PAP/ACP5 Antibodies
SKU/Catalog Number
A03277
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TRAP ACP5 Antibody (Catalog # A03277). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
TRAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-TRAP ACP5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03277)
Host
Rabbit
Contents
TRAP Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TRAP antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human TRAP. The immunogen is located within the last 50 amino acids of TRAP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of TRIM24 are known to exist.
Reactive Species
A03277 is reactive to ACP5 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
36599 MW
Background of TRACP/PAP/ACP5
TRAP, also known as uteroferrin, is an iron containing, glycosylated, acid phosphatase. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. Along with the related protein ACP2, TRAP mediates the removal of mannose 6-phosphate residues from proteins targeted to lysosomes. TRAP is present in brain at low levels, but is expressed at a much higher level in liver.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03277 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TRAP antibody can be used for detection of TRAP by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of TRAP in mouse brain tissue lysate with TRAP antibody at 1 μg/mL.
Click image to see more details
Immunofluorescence of TRAP in human liver tissue with TRAP antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ACP5 (Source: Uniprot.org, NCBI)
Gene Name
ACP5
Full Name
Tartrate-resistant acid phosphatase type 5
Weight
36599 MW
Superfamily
metallophosphoesterase superfamily
Alternative Names
TRAP, SPENCDI, Tartrate-resistant acid phosphatase type 5, Tartrate-resistant acid ATPase, TR-AP ACP5 HPAP, TRACP5a, TRACP5b, TRAP, TrATPase acid phosphatase 5, tartrate resistant tartrate-resistant acid phosphatase type 5|human purple acid phosphatase|tartrate-resistant acid ATPase|tartrate-resistant acid phosphatase 5a|tartrate-resistant acid phosphatase 5b
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACP5, check out the ACP5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACP5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRAP ACP5 Antibody (A03277)
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