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Product Info Summary
| SKU: | A05906 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Translocation protein SEC62 SEC62 Antibody
SKU/Catalog Number
A05906
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Translocation protein SEC62 SEC62 Antibody (Catalog # A05906). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SEC62 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Translocation protein SEC62 SEC62 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05906)
Host
Rabbit
Contents
SEC62 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SEC62 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SEC62. The immunogen is located within amino acids 270 - 320 of SEC62.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
SEC62 antibody is predicted to not cross-react with other SEC proteins. Multiple isoforms of SEC62 are known to exist.
Reactive Species
A05906 is reactive to SEC62 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
45862 MW
Background of SEC62
SEC62 is an integral membrane protein located in the rough endoplasmic reticulum (ER) and is part of the SEC61-SEC62-SEC63 complex that is the central component of the protein translocation apparatus of the ER membrane. It is speculated that SEC61-SEC62-SEC63 may perform post-translational protein translocation into the ER and might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. Silencing of this gene with RNAi dramatically reduced the migration and invasive potential of numerous tumor cell lines, suggesting that it may be an attractive target for therapy of various tumors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05906 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SEC62 antibody can be used for detection of SEC62 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SEC62 in rat brain tissue lysate with SEC62 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunohistochemistry of SEC62 in mouse brain tissue with SEC62 antibody at 5 μg/mL.
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Immunofluorescence of SEC62 in mouse brain tissue with SEC62 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For SEC62 (Source: Uniprot.org, NCBI)
Gene Name
SEC62
Full Name
Translocation protein SEC62
Weight
45862 MW
Superfamily
SEC62 family
Alternative Names
HTP1, TP-1, Dtrp1, TLOC1, Translocation protein SEC62, Translocation protein 1 SEC62 Dtrp1, HTP1, TLOC1, TP-1 SEC62 homolog, preprotein translocation factor translocation protein SEC62|SEC62 preprotein translocation factor|hTP-1|membrane protein SEC62, S.cerevisiae, homolog of|translocation protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SEC62, check out the SEC62 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SEC62: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Translocation protein SEC62 SEC62 Antibody (A05906)
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No publications found for A05906
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