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Product Info Summary
| SKU: | A00662 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Transcription factor EB TFEB Antibody
SKU/Catalog Number
A00662
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Transcription factor EB TFEB Antibody (Catalog # A00662). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
TFEB antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Transcription factor EB TFEB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00662)
Host
Rabbit
Contents
TFEB Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TFEB antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human TFEB. The immunogen is located within amino acids 70 - 120 of TFEB.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two antibodies are known to exist; this antibody will detect both isoforms.
Reactive Species
A00662 is reactive to TFEB in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
52865 MW
Background of TFEB
The Transcription factor EB (TFEB) is a member in the basic helix-loop-helix leucine zipper superfamily of transcription factors that is translocated in a subset of renal tumors. Recent studies have shown that lysosomal biogenesis is regulated by TFEB, which is in turn regulated by the mammalian target of rapamycin (mTOR) complex 1. Other evidence suggests that TFEB coordinates the major steps of the autophagic pathway by driving the expression of autophagy and lysosomal genes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00662 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TFEB antibody can be used for detection of TFEB by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TFEB in A549 cell lysate with TFEB antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of TFEB (NT) in human lung tissue with TFEB (NT) antibody at 2.5 μg/mL.
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Immunofluorescence of TFEB in human lung tissue with TFEB antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For TFEB (Source: Uniprot.org, NCBI)
Gene Name
TFEB
Full Name
Transcription factor EB
Weight
52865 MW
Superfamily
MiT/TFE family
Alternative Names
TCFEB, BHLHE35, ALPHATFEB, Transcription factor EB, Class E basic helix-loop-helix protein 35, bHLHe35 TFEB ALPHATFEB, BHLHE35, TCFEB transcription factor EB transcription factor EB|T-cell transcription factor EB|class E basic helix-loop-helix protein 35
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TFEB, check out the TFEB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TFEB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transcription factor EB TFEB Antibody (A00662)
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