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Product Info Summary
| SKU: | A02592 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
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Product info
Product Name
Anti-Transcription factor 12 TCF12 Antibody
View all TCF-12/HTF4 Antibodies
SKU/Catalog Number
A02592
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Transcription factor 12 TCF12 Antibody (Catalog # A02592). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TCF12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Transcription factor 12 TCF12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02592)
Host
Rabbit
Contents
TCF12 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TCF12 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TCF12. The immunogen is located within amino acids 50 - 100 of TCF12.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of SATB2 are known to exist. This SATB2 antibody will not cross-react with SATB1.
Reactive Species
A02592 is reactive to TCF12 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
72965 MW
Background of TCF-12/HTF4
TCF12, also known as HTF4, is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. TCF12 is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. TCF12, in combination with E2A, is required to block thymocyte proliferation prior to pre-TCR expression and is critical for proper T cell differentiation. Recent reports have shown that TCF12 is also a critical factor required for the development of invariant natural killer T cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02592 is guaranteed for ELISA, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TCF12 antibody can be used for detection of TCF12 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TCF12 in HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunocytochemistry of TCF12 in HeLa cells with TCF12 antibody at 10 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For TCF12 (Source: Uniprot.org, NCBI)
Gene Name
TCF12
Full Name
Transcription factor 12
Weight
72965 MW
Alternative Names
HEB, CRS3, HTF4, bHLHb20, HsT17266, BHLHB20, HEB, Transcription factor 12, Class B basic helix-loop-helix protein 20, TCF-12 TCF12 CRS3, HEB, HTF4, HsT17266, TCF-12, bHLHb20, p64 transcription factor 12 transcription factor 12|DNA-binding protein HTF4|E-box-binding protein|class B basic helix-loop-helix protein 20|helix-loop-helix transcription factor 4|transcription factor HTF-4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TCF12, check out the TCF12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TCF12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transcription factor 12 TCF12 Antibody (A02592)
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