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Product Info Summary
| SKU: | PA2009 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-TRAF4 Antibody Picoband®
SKU/Catalog Number
PA2009
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRAF4 Antibody catalog # PA2009. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRAF4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2009)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TRAF4, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2009 is reactive to TRAF4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
56 kDa
Calculated molecular weight
53543 MW
Background of TRAF-4
TRAF4 (TNF Receptor-Associated Factor 4), also called CART1 or MLN62, is a protein that in humans is encoded by the TRAF3 gene. TRAF4 is a member of the TNF receptor associated factor (TRAF) family, a family of scaffold proteins. Tomasetto et al. (1995) identified genes that are overexpressed in breast cancer by differential screening of cDNAs from breast cancer-derived metastatic axillary lymph nodes. The TRAF4 cDNA, which they designated MLN62, was mapped to 17q11-q12 by radioisotopic in situ hybridization. To investigate the biologic function of TRAF4, Regnier et al. (2002) generated Traf4-deficient mice by gene disruption.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2009 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Thymus Tissue, HELA Cell, JURKAT Cell, HEPA Cell,
IHC: Rat Lymphonodus Tissue, Human Lung Cancer Tissue
Validation Images & Assay Conditions
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Anti-TRAF4 antibody, PA2009, Western blotting
Lane 1: Rat Thymus Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: JURKAT Cell Lysate
Lane 4: HEPA Cell Lysate
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Anti-TRAF4 antibody, PA2009, IHC(P)
IHC(P): Rat Lymphonodus Tissue
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Anti-TRAF4 antibody, PA2009, IHC(P)
IHC(P): Human Lung Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For TRAF4 (Source: Uniprot.org, NCBI)
Gene Name
TRAF4
Full Name
TNF receptor-associated factor 4
Weight
53543 MW
Superfamily
TNF receptor-associated factor family
Alternative Names
TNF receptor-associated factor 4;Cysteine-rich domain associated with RING and Traf domains protein 1;Metastatic lymph node gene 62 protein;MLN 62;RING finger protein 83;TRAF4;CART1, MLN62, RNF83; TRAF4 CART1, MLN62, RNF83 TNF receptor associated factor 4 TNF receptor-associated factor 4|MLN 62|RING finger protein 83|TRAF4 variant 6|cysteine-rich domain associated with RING and Traf domains protein 1|cysteine-rich domain associated with ring and TRAF domain|malignant 62|metastatic lymph node gene 62 protein|tumor necrosis receptor-associated factor 4A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRAF4, check out the TRAF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRAF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRAF4 Antibody Picoband® (PA2009)
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No publications found for PA2009
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Customer Q&As
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1 Customer Q&As for Anti-TRAF4 Antibody Picoband®
Question
We are currently using anti-TRAF4 antibody PA2009 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
B. Zhang
Verified customer
Asked: 2016-02-26
Answer
The anti-TRAF4 antibody (PA2009) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-02-26
