Click image to see more details
Product Info Summary
| SKU: | A01704-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CEP68 Antibody Picoband®
SKU/Catalog Number
A01704-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CEP68 Antibody Picoband® catalog # A01704-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CEP68 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01704-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CEP68, which shares 73.2% and 75.6% amino acid (aa) sequence identity with mouse and rat EP68, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01704-1 is reactive to CEP68 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
81 kDa
Calculated molecular weight
81.102kDa
Background of CEP68
Centrosomal protein of 68 kDa is a protein that in humans is encoded by the CEP68 gene. It is mapped to chromosome 2. CEP68 is required for centrosome cohesion. It decorates fibres emanating from the proximal ends of centrioles. CEP68 and rootletin depend both on each other for centriole association, and both also require CEP250 for their function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01704-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: human Hela whole cell, human COLO-320 whole cell, human SK-OV-3 whole cell, human Jurkat whole cell, rat heart tissue, mouse heart tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CEP68 using anti-CEP68 antibody (A01704-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human COLO-320 whole cell lysates,
Lane 3: human SK-OV-3 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: mouse heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEP68 antigen affinity purified polyclonal antibody (Catalog # A01704-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEP68 at approximately 81KD. The expected band size for CEP68 is at 81KD.
Protein Target Info & Infographic
Gene/Protein Information For CEP68 (Source: Uniprot.org, NCBI)
Gene Name
CEP68
Full Name
Centrosomal protein of 68 kDa
Weight
81.102kDa
Alternative Names
Centrosomal protein of 68 kDa; Cep68; CEP68; KIAA0582 CEP68 KIAA0582 centrosomal protein 68 centrosomal protein of 68 kDa|centrosomal protein 68kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CEP68, check out the CEP68 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CEP68: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CEP68 Antibody Picoband® (A01704-1)
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Customer Q&As
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4 Customer Q&As for Anti-CEP68 Antibody Picoband®
Question
Do you have a BSA free version of anti-CEP68 antibody A01704-1 available?
P. Gonzalez
Verified customer
Asked: 2020-02-06
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CEP68 antibody A01704-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-06
Question
We are currently using anti-CEP68 antibody A01704-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
The anti-CEP68 antibody (A01704-1) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-21
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-CEP68 antibody A01704-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-07-26
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-26
Question
Does anti-CEP68 antibody A01704-1 work for WB with brain?
L. Moore
Verified customer
Asked: 2016-02-08
Answer
According to the expression profile of brain, CEP68 is highly expressed in brain. So, it is likely that anti-CEP68 antibody A01704-1 will work for WB with brain.
Boster Scientific Support
Answered: 2016-02-08
