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Product Info Summary
| SKU: | M02785 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TRADD Monoclonal Antibody
SKU/Catalog Number
M02785
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TRADD Monoclonal Antibody catalog # M02785. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRADD Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02785)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADCA-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TRADD Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02785 is reactive to TRADD in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
70 kDa, 90 kDa
Calculated molecular weight
98399 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02785 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:10
Positive Control
WB: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of TRADD expression in Hela cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For TRADD (Source: Uniprot.org, NCBI)
Gene Name
TRADD
Full Name
Tumor necrosis factor receptor type 1-associated DEATH domain protein
Weight
98399 MW
Alternative Names
Cytoplasmic aconitate hydratase;Aconitase;4.2.1.3;Citrate hydro-lyase;Ferritin repressor protein;Iron regulatory protein 1;IRP1;Iron-responsive element-binding protein 1;IRE-BP 1;ACO1;IREB1; TRADD Hs.89862 TNFRSF1A associated via death domain tumor necrosis factor receptor type 1-associated DEATH domain protein|TNFR1-associated death domain protein|tumor necrosis factor receptor type 1 associated death domain protein|tumor necrosis factor receptor-1-associated protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRADD, check out the TRADD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRADD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRADD Monoclonal Antibody (M02785)
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No publications found for M02785
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-TRADD Monoclonal Antibody
Question
Does anti-TRADD Monoclonal antibody M02785 work on zebrafish IF with pancreas?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
Our lab technicians have not tested anti-TRADD Monoclonal antibody M02785 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-TRADD Monoclonal antibody M02785 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish pancreas in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-07
Question
Will M02785 anti-TRADD Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
It shows on the product datasheet, M02785 anti-TRADD Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-09
Question
We are currently using anti-TRADD Monoclonal antibody M02785 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-02-05
Answer
The anti-TRADD Monoclonal antibody (M02785) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-05
Question
Is a blocking peptide available for product anti-TRADD Monoclonal antibody (M02785)?
K. Mangal
Verified customer
Asked: 2018-11-16
Answer
We do provide the blocking peptide for product anti-TRADD Monoclonal antibody (M02785). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-11-16
Question
I have a question about product M02785, anti-TRADD Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Walker
Verified customer
Asked: 2018-08-23
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02785 anti-TRADD Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-23
Question
Is this M02785 anti-TRADD Monoclonal antibody reactive to the isotypes of TRADD?
Verified Customer
Verified customer
Asked: 2017-08-14
Answer
The immunogen of M02785 anti-TRADD Monoclonal antibody is A synthesized peptide derived from human TRADD Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-08-14
