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Product Info Summary
| SKU: | A13140 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-STON1/Stonin 1 Antibody
SKU/Catalog Number
A13140
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-STON1/Stonin 1 Antibody (Catalog # A13140). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-STON1/Stonin 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13140)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human STON1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A13140 is reactive to STON1 in Human
Reconstitution
Calculated molecular weight
83141 MW
Background of Stonin-1
May be involved in the endocytic machinery By similarity.
Martina J.A., J. Cell Biol. 153:1111-1120(2001).
Han S., Biol. Reprod. 64:507-517(2001).
Upadhyaya A.B., J. Biol. Chem. 274:18040-18048(1999).<
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13140 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from K562 cells, using STON1 antibody A13140.
Protein Target Info & Infographic
Gene/Protein Information For STON1 (Source: Uniprot.org, NCBI)
Gene Name
STON1
Full Name
Stonin-1
Weight
83141 MW
Superfamily
Stoned B family
Alternative Names
Stonin-1;Stoned B-like factor;STON1;SALF, SBLF, STN1; STON1 SALF, SBLF, STN1, STNB1 stonin 1 stonin-1|stoned B homolog 1|stoned B-like factor|stoned-b1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STON1, check out the STON1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STON1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-STON1/Stonin 1 Antibody (A13140)
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No publications found for A13140
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-STON1/Stonin 1 Antibody
Question
We are currently using anti-STON1/Stonin 1 antibody A13140 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
The anti-STON1/Stonin 1 antibody (A13140) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-14
Question
Is there a BSA free version of anti-STON1/Stonin 1 antibody A13140 available?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-STON1/Stonin 1 antibody A13140 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-29
Question
I was wanting to use your anti-STON1/Stonin 1 antibody for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
It shows on the product datasheet, A13140 anti-STON1/Stonin 1 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-24
Question
Would A13140 anti-STON1/Stonin 1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-08-22
Answer
As indicated on the product datasheet, A13140 anti-STON1/Stonin 1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-22
Question
My question regarding product A13140, anti-STON1/Stonin 1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-03-20
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13140 anti-STON1/Stonin 1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-03-20
