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Product Info Summary
| SKU: | M00953 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal Antibody
SKU/Catalog Number
M00953
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal Antibody (Catalog # M00953). Tested in IF, WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00953)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: TOP2A/1361
Isotype
IgG2b, kappa
Immunogen
Recombinant human Topoisomerase II alpha fragment (aa1352-1493)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M00953 is reactive to TOP2A in Human, Mouse
Applications
M00953 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
174385 MW
Background of TOP2A
This monoclonal antibody recognizes a 170kDa protein, which is identified as topoisomerase II is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti- Topoisomerase II alpha Monoclonal Antibody (TOP2A/1361).
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Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Anti-Topoisomerase II alpha Monoclonal Antibody (TOP2A/1361).
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Western Blot of HepG2, HeLa and 3T3 cell lysate using Anti-Topo II alpha, Monoclonal Antibody (TOP2A/1361).
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Western Blot analysis of human HeLa cell lysate using Anti-Topoisomerase II alpha, Monoclonal Antibody (TOP2A/1361).
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Confocal Immunofluorescence image of HeLa cells using Anti-Topo II alpha, Monoclonal Antibody (TOP2A/1361). Green (CF488) and Phalloidin (Red) are used to label the nuclei.
Protein Target Info & Infographic
Gene/Protein Information For TOP2A (Source: Uniprot.org, NCBI)
Gene Name
TOP2A
Full Name
DNA topoisomerase 2-alpha
Weight
174385 MW
Superfamily
type II topoisomerase family
Alternative Names
DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase 2-alpha; DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; EC 5.99.1.3; TOP2; TOP2A; TOP2DNA gyrase; topoisomerase (DNA) II alpha (170kD); topoisomerase (DNA) II alpha 170kDa; TP2A TOP2A TOP2, TP2A DNA topoisomerase II alpha DNA topoisomerase 2-alpha|DNA gyrase|DNA topoisomerase (ATP-hydrolyzing)|DNA topoisomerase II, 170 kD|DNA topoisomerase II, alpha isozyme|topoisomerase (DNA) II alpha 170kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TOP2A, check out the TOP2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TOP2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal Antibody (M00953)
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No publications found for M00953
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal Antibody
Question
We have seen staining in human ovarian carcinoma. Are there any suggestions? Is anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody supposed to stain ovarian carcinoma positively?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
According to literature ovarian carcinoma does express TOP2A. According to Uniprot.org, TOP2A is expressed in heart, ovarian carcinoma, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have TOP2A expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 10942766, 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2020-04-29
Question
I have a question about product M00953, anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Johnson
Verified customer
Asked: 2020-03-11
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00953 anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-11
Question
I was wanting to use to test anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody M00953 on mouse cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody M00953 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Baker
Verified customer
Asked: 2020-02-24
Answer
The products we sell, including anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody M00953, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-24
Question
I see that the anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody M00953 works with IHC, what is the protocol used to produce the result images on the product page?
B. Krishna
Verified customer
Asked: 2020-01-30
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-30
Question
Is a blocking peptide available for product anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody (M00953)?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
We do provide the blocking peptide for product anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody (M00953). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-30
Question
Would anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody M00953 work on pig IHC with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
Our lab technicians have not validated anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody M00953 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody M00953 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-06
Question
Is this M00953 anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody reactive to the isotypes of TOP2A?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
The immunogen of M00953 anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody is Recombinant human Topoisomerase II alpha fragment (aa1352-1493) (exact sequence is proprietary). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-02
Question
Will M00953 anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
You can see on the product datasheet, M00953 anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-18
Question
Our team were well pleased with the WB result of your anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody. However we have observed positive staining in cervix carcinoma cytoplasm. using this antibody. Is that expected? Could you tell me where is TOP2A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
Based on literature, cervix carcinoma does express TOP2A. Generally TOP2A expresses in cytoplasm. Regarding which tissues have TOP2A expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 10942766, 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2019-06-26
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody M00953. Let me know if you need anything else.
W. Banerjee
Verified customer
Asked: 2019-05-21
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-21
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody M00953. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-01-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-29
Question
Do you have a BSA free version of anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody M00953 available?
F. Wu
Verified customer
Asked: 2017-03-07
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody M00953 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-03-07
Question
Will anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody M00953 work for IHC with cervix carcinoma erythroleukemia?
G. Yang
Verified customer
Asked: 2014-12-30
Answer
According to the expression profile of cervix carcinoma erythroleukemia, TOP2A is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody M00953 will work for IHC with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2014-12-30
Question
I was wanting to use your anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody for IHC for mouse cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma erythroleukemia identification?
A. Li
Verified customer
Asked: 2013-09-03
Answer
It shows on the product datasheet, M00953 anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody has been validated for IHC on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-09-03
Question
We are currently using anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody M00953 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on zebrafish tissues as well?
K. Roberts
Verified customer
Asked: 2013-08-26
Answer
The anti-Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Monoclonal antibody (M00953) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-08-26
