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Product Info Summary
| SKU: | A02750 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-TOP2B/Topoisomerase Ii Beta Antibody
SKU/Catalog Number
A02750
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TOP2B/Topoisomerase Ii Beta Antibody (Catalog# A02750). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TOP2B/Topoisomerase Ii Beta Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02750)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human TOP2B. Immunogen sequence location: 1-50
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02750 is reactive to TOP2B in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
183267 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02750 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from Jurkat cells, using TOP2B Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using TOP2B Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For TOP2B (Source: Uniprot.org, NCBI)
Gene Name
TOP2B
Full Name
DNA topoisomerase 2-beta
Weight
183267 MW
Superfamily
type II topoisomerase family
Alternative Names
TOP2B;DNA topoisomerase 2-beta;DNA topoisomerase II; beta isozyme TOP2B TOPIIB, top2beta DNA topoisomerase II beta DNA topoisomerase 2-beta|DNA topoisomerase II, 180 kD|DNA topoisomerase II, beta isozyme|U937 associated | MLAA-44|topo II beta|topoisomerase (DNA) II beta 180kDa|topoisomerase II beta|topoisomerase IIb
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TOP2B, check out the TOP2B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TOP2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TOP2B/Topoisomerase Ii Beta Antibody (A02750)
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No publications found for A02750
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-TOP2B/Topoisomerase Ii Beta Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-TOP2B/Topoisomerase Ii Beta antibody A02750. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-18
Question
Would anti-TOP2B/Topoisomerase Ii Beta antibody A02750 work for IHC with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
According to the expression profile of leukemic t-cell, TOP2B is highly expressed in leukemic t-cell. So, it is likely that anti-TOP2B/Topoisomerase Ii Beta antibody A02750 will work for IHC with leukemic t-cell.
Boster Scientific Support
Answered: 2019-12-16
Question
Please see the WB image, lot number and protocol we used for leukemic t-cell using anti-TOP2B/Topoisomerase Ii Beta antibody A02750. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-12
Question
We are currently using anti-TOP2B/Topoisomerase Ii Beta antibody A02750 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-08
Answer
The anti-TOP2B/Topoisomerase Ii Beta antibody (A02750) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-08
