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Product Info Summary
| SKU: | M08668 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TOMM22/TOM22 Rabbit Monoclonal Antibody
SKU/Catalog Number
M08668
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-TOMM22/TOM22 Rabbit Monoclonal Antibody catalog # M08668. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TOMM22/TOM22 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08668)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
22T35
Isotype
IgG
Immunogen
A synthesized peptide derived from human TOMM22
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M08668 is reactive to TOMM22 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
18 kDa
Calculated molecular weight
50152 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08668 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: human Hela whole cell, human 293T whole cell, human MCF-7 whole cell, human K562 whole cell, human HepG2 whole cell, human SK-OV-3 whole cell, human Jurkat whole cell, human Raji whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CLIC4 using anti-CLIC4 antibody (M08668).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human SK-OV-3 whole cell lysates,
Lane 7: human Jurkat whole cell lysates,
Lane 8: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLIC4 antigen affinity purified monoclonal antibody (Catalog # M08668) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLIC4 at approximately 18 kDa. The expected band size for CLIC4 is at 29 kDa.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
Protein Target Info & Infographic
Gene/Protein Information For TOMM22 (Source: Uniprot.org, NCBI)
Gene Name
TOMM22
Full Name
Mitochondrial import receptor subunit TOM22 homolog
Weight
50152 MW
Superfamily
Tom22 family
Alternative Names
Tubulin alpha-1B chain;Alpha-tubulin ubiquitous;Tubulin K-alpha-1;Tubulin alpha-ubiquitous chain;TUBA1B; TOMM22 1C9-2, MST065, MSTP065, TOM22 translocase of outer mitochondrial membrane 22 mitochondrial import receptor subunit TOM22 homolog|mitochondrial import receptor Tom22|translocase of outer membrane 22 kDa subunit homolog|translocase of outer mitochondrial membrane 22 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TOMM22, check out the TOMM22 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TOMM22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TOMM22/TOM22 Rabbit Monoclonal Antibody (M08668)
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