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Product Info Summary
| SKU: | A06499-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-TNNI3K Antibody Picoband®
SKU/Catalog Number
A06499-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TNNI3K Antibody Picoband® catalog # A06499-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TNNI3K Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06499-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TNNI3K recombinant protein (Position: F102-Q783).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06499-2 is reactive to TNNI3K in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
110 kDa
Calculated molecular weight
92.851kDa
Background of TNNI3K
TNNI3 interacting kinase is a protein that in humans is encoded by the TNNI3K gene. This gene encodes a protein that belongs to the MAP kinase kinase kinase (MAPKKK) family of protein kinases. The protein contains ankyrin repeat, protein kinase and serine-rich domains and is thought to play a role in cardiac physiology.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06499-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat heart tissue, mouse heart tissue
FCM: HEL cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TNNI3K using anti-TNNI3K antibody (A06499-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNNI3K antigen affinity purified polyclonal antibody (Catalog # A06499-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNNI3K at approximately 110 kDa. The expected band size for TNNI3K is at 93 kDa.
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Figure 2. Flow Cytometry analysis of HEL cells using anti-TNNI3K antibody (A06499-2).
Overlay histogram showing HEL cells stained with A06499-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TNNI3K Antibody (A06499-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For TNNI3K (Source: Uniprot.org, NCBI)
Gene Name
TNNI3K
Full Name
Serine/threonine-protein kinase TNNI3K
Weight
92.851kDa
Superfamily
protein kinase superfamily
Alternative Names
Max dimerization protein 1; Max dimerizer 1; Protein MAD; MXD1; MAD TNNI3K CARK, CCDD TNNI3 interacting kinase serine/threonine-protein kinase TNNI3K|cardiac ankyrin repeat kinase|cardiac troponin I-interacting kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNNI3K, check out the TNNI3K Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNNI3K: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TNNI3K Antibody Picoband® (A06499-2)
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